“…They demonstrated that when the intracellular Ca 2+ concentration was increased by treatment with A23187, a bivalent cation ionophore, there was a shape change from discocytes to echinocytes, the 1,2‐diacylglycerol content of the membranes increased, and microvesicles budded from the microvillous‐like projections of the echinocytes. These vesicles were depleted in phosphatidylcholine, spectrin, actin (band 5), and glycophorin, and band 3 and acetylcholinesterase (AChE) were retained 34,58‐61 …”