DNA damage response is a key signal transduction pathway in triggering ageing and tumor progression. Abnormal alternative splicing (AS) is associated with tumors and ageing. However, the role of AS factors associated with DNA damage repair and ageing in stomach adenocarcinoma (STAD) remains unclear. We downloaded the percentage of splicing (PSI) values for AS in STAD from the TCGA SpliceSeq database. The PSI values of DNA repair gene AS events were integrated with STAD patient survival data for Cox regression analysis. The prediction model for the overall survival (OS) was constructed by the clinical traits. The tumor immune microenvironment was analyzed by CIBERSORT and ESTIMATE. We detected 824 AS events originating from 166 DNA repair genes. Cox regression analysis provided 21 prognostic AS events connected with OS statistically, and a prognostic prediction model was constructed. The expression of these AS factors was higher in STAD tumors. DDB2 high senescence levels were associated with active immune responses and better survival in STAD patients. We built a novel prognostic model founded on DNA repair genes with AS events and identified that DDB2 may be a potential biomarker to apply in clinics.
As oil exploitation enters its middle and late stages, formation pressure drops, and crude oil degases. In production profile logging, the presence of the gas phase will affect the initial oil–water two-phase flowmeter’s flow measurement results. In order to eliminate gas-phase interference and reduce measurement costs, we designed a downhole gas–liquid separator (DGLS) suitable for low flow, high water holdup, and high gas holdup. We based it on the phase isolation method. Using a combination of numerical simulation and fluid dynamic measurement experiments, we studied DGLS separation efficiency separately in the two cases of gas–water two-phase flow and oil–gas–water three-phase flow. Comparative analysis of the numerical simulation calculation and dynamic test results showed that: the VOF model constructed based on k−ε the equation is nearly identical to the dynamic test, and can be used to analyze DGLS separation efficiency; the numerical simulation results of the gas–water two-phase flow show that when the total flow rate is below 20 m3/d, the separation efficiency surpasses 90%. The oil–gas–water three-phase’s numerical simulation results show that the oil phase influences separation efficiency. When the total flow rate is 20 m3/d–50 m3/d and gas holdup is low, the DGLS’s separation efficiency can exceed 90%. Our experimental study on fluid dynamics measurement shows that the DGLS’s applicable range is when the gas mass is 0 m3/d~15 m3/d, and the water holdup range is 50%~100%. The research presented in this article can provide a theoretical basis for the development and design of DGLSs.
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