The aim of this article is to provide the detailed study of the changes in the lactobacilli indices of the oral biotope in health and in caries progression in individuals. Materials and methods: Materials included on reports and data of research projects conducted at the Department of Therapeutic Dentistry and Department of Microbiology, Virology and Immunology, Ukrainian Medical Stomatological Academy (Poltava), at the Department of Microbiology of Danylo Halytsky National Medical University (Lviv), completed in 2013;research data documented by the Department of Microbiology, Medical Stomatological Institute (now Ukrainian Medical Stomatological Academy, Poltava) in 1978; literature related to the subject matter. Methodology: the standard methods of dental examination recommended by WHO; methods of system analysis and system approach, bibliosemantic analysis . Results: The presented results indicate that the number of representatives of different types of oral microflora in the oral microbiocenosis is different at different stages of human life. The content of Lactobacilli in children decreases following the rise even under increased dmft indices. Conclusions: The data observed are of great clinical interest when considering the role of Lactobacilli in the protection of the host organism and the initiation of the carious process.

The aim: Calculate the antagonistic activity of representatives of the oral biotope. Materials and methods: The mathematical techniques used in biotechnology and dentistry. The calculations were carried out on the basis of the research data obtained by the investigators from Ukrainian Medical Stomatological Academy (UMSA) (Poltava). Results: The paper shows that the oral biocenosis is a group of microorganisms that have similar characteristics within the group, but different outside. This may indicate to the relationships between the bacteria, existing within such groups. A graphical demonstration of the associates of the oral biotope with different DMF represents their growth phases depending on the intensity of dental caries. The different slope and phases of the logistic curve of their growth may indicate certain relationships arising between groups of microorganisms. Conclusions: The symbiotic relationships within the oral biocenosis can be manifested by the antagonism, as indicated by the index of antagonistic activity, which in most cases can be calculated. Antagonistic activity can be determined more precisely, for which special techniques have been developed. Antagonistic activity of associates of biocenosis can serve as an indicator of the “conditionally pathogenic - pathogenic” transformation. Data on antagonistic activity can be used in oral hygiene activities and treatment of dental caries to impact the oral microbiocenosis. Data on antagonistic activity may have a predictive value.

The aim of the present research project was to study the effect of ultrasonic waves on the S. epidermidis ATCC 14990 museum culture. Materials and methods: A bibliosemantic method – for analyzing previous research based on scientific literature, electronic resources, empirical methods of scientific research (observation, comparison, measurement, experiment) – to study the effect of ultrasound on the strain of S. epidermidis ATCC 14990. Results: A study showed that when exposed to ultrasound for four and a half minutes on a culture of microorganisms S. epidermidis in glass tubes, the number of viable cells in the suspension decreases. Conclusions: The influence of ultrasound on the culture of S. epidermidis, studied in the work, showed that the selected and used parameters of ultrasound treatment cause destructive changes in microorganisms. These changes are not fatal for the entire bacterial culture used in the study, but they have a reliable antimicrobial effect.