The aim of the present research project was to study the effect of ultrasonic waves on the S. epidermidis ATCC 14990 museum culture. Materials and methods: A bibliosemantic method – for analyzing previous research based on scientific literature, electronic resources, empirical methods of scientific research (observation, comparison, measurement, experiment) – to study the effect of ultrasound on the strain of S. epidermidis ATCC 14990. Results: A study showed that when exposed to ultrasound for four and a half minutes on a culture of microorganisms S. epidermidis in glass tubes, the number of viable cells in the suspension decreases. Conclusions: The influence of ultrasound on the culture of S. epidermidis, studied in the work, showed that the selected and used parameters of ultrasound treatment cause destructive changes in microorganisms. These changes are not fatal for the entire bacterial culture used in the study, but they have a reliable antimicrobial effect.

The aim of this article is to provide the detailed study of the changes in the lactobacilli indices of the oral biotope in health and in caries progression in individuals. Materials and methods: Materials included on reports and data of research projects conducted at the Department of Therapeutic Dentistry and Department of Microbiology, Virology and Immunology, Ukrainian Medical Stomatological Academy (Poltava), at the Department of Microbiology of Danylo Halytsky National Medical University (Lviv), completed in 2013;research data documented by the Department of Microbiology, Medical Stomatological Institute (now Ukrainian Medical Stomatological Academy, Poltava) in 1978; literature related to the subject matter. Methodology: the standard methods of dental examination recommended by WHO; methods of system analysis and system approach, bibliosemantic analysis . Results: The presented results indicate that the number of representatives of different types of oral microflora in the oral microbiocenosis is different at different stages of human life. The content of Lactobacilli in children decreases following the rise even under increased dmft indices. Conclusions: The data observed are of great clinical interest when considering the role of Lactobacilli in the protection of the host organism and the initiation of the carious process.

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Fixed orthodontic appliances are widely used in the correction of dentoalveolar anomalies. Examinations prior the beginning of orthodontic treatment revealed numerous cases of dental caries of moderate intensity; moreover, caries prevalence increases with age that is the number of carious teeth goes up, while the number of filled teeth and the level of oral hygiene become lower. A voluminous body of reports indicates that children and adolescents wearing orthodontic appliances have a significantly higher incidence of caries than patients who do not wear them. Plaque microflora is one of the main risk factors for the development of caries. According to the researchers, caries is initiated by streptococci, lactobacilli, actinomycetes, and Candida fungi. To assess the content of cariogenic microorganisms (Streptococcus mutans and Lactobacillus), there are special microbiological tests available. Kits for rapid tests are manufactured in different countries around the world. In 2013, the researchers of the Department of Therapeutic Dentistry and the Department of Microbiology, Virology and Immunology, Ukrainian Medical Stomatological Academy investigated the microbiocenosis in the oral cavity in young people aged 19-25 years with different intensity of the carious process. The percentage and quantitative content of microflora was studied. The results of the study showed that an increase in the intensity of dental caries is accompanied by changes in the balance in the composition of the oral microflora. This study elaborated the indicators for identifying lactobacilli, streptococci (including haemolytic streptococci, which include S. mutans), fungi of the genus Candida and actinomycetes. But the results obtained did not match the application range of the above mentioned tests. It means the tests reflecting the number of representatives of the studied microbiocenosis in order to determine their activity, are not sufficiently objective. However, rapid tests assessing the indices of cariogenic microflora are in demand in dental practice that allows us to recommend their application in orthodontics. Further study of microbiocenoses should be carried out to determine their functional properties.