Abstract. Our interest to search for anticancer bioactive compounds from Benalu Batu (Begonia sp), we applied molecular docking approach to identify the natural product compounds that might be responsible for anticancer activity with a specific target and selective inhibition mechanism. Our goal is to identify the potential anticancer compounds based on virtual screening on the protein of Epidermal Growth Factor Receptor Tyrosine Kinase (EGFR-TK) as virtual inhibition target from reported chemical constituents of Begonia species. Molecular docking was performed by using open babel, SPORES, and PLANTS1.2 software under Fedora Linux operation system and validated based on Root Mean Square Deviation (RMSD) value. The 62 compounds from 9 species of Begonia were docked to the pocket of erlotinib binding site in EGFR-TK protein. The docking result showed that the compound type of alkaloid, steroidal glycoside, triterpenoid glycoside and flavonoid glycoside (polyphenol) have higher chemPLP docking score than other compounds and co-crystallized ligand erlotinib. This result suggested the high potential anticancer activity of alkaloid and glycoside type compounds from Begonia species that lead us to identify and isolate these types of compounds from Begonia sp.
Hantap (Sterculia coccinea Jack) merupakan salah satu tumbuhan di Sulawesi Tengah yang mempunyai potensi sebagai antioksidan sehingga dapat dimanfaatkan untuk mengobati berbagai macam penyakit. Penelitian ini bertujuan untuk menentukan nilai IC50 ekstrak etanol daun hantap dengan metode DPPH dan LC50 dengan metode BSLT serta mengetahui kandungan kimianya.Ekstrak dibuat dengan metode maserasi menggunakan pelarut etanol 96%.Penentuan kandungan kimia dilakukan dengan menggunakan pereaksi identifikasi dankromatografi lapis tipis (KLT).Uji aktivitas antioksidan ekstrak etanol daun hantap diperoleh nilai IC50 sebesar 6,48 ppm dan vitamin C (kontrol positif) 2,71 ppm, sedangkan aktivitas sitotoksiknya dihasilkan nilai LC50 sebesar 591,561 ppm. Hasil identifikasi dengan pereaksi identifikasi dan KLT menunjukkan ekstrak mengandung flavonoid, fenol, saponin, steroid, alkaloid, dan tanin. Berdasarkan hasil uji, ekstrak etanol daun hantap dapat dikatakan memiliki aktivitas antioksi dan yang sangat kuat, sedangkan aktivitas sitotoksiknya masuk kategori sedang.
Pepolo (Bischofia javanica Blume) is an Indonesian native plant whose stem bark is widely used as a traditional medicine for various diseases. Therefore, this study aims to determine the chemical profile of the secondary metabolites of the Pepolo stem bark (PSB) extract and its effectiveness as a potential immunomodulatory and anticancer agent. The PSBs were extracted by a reflux technique with 96% v/v ethanol as solvent. The immunomodulatory activity of the PSB extract was evaluated based on its effect on the macrophages of BALB/C mice, while the cytotoxicity and anticancer potential were observed as the PSB extract response on brine shrimp lethality test and MCF-7 cancer cells, respectively. The chemical profiles of the secondary metabolites were analyzed using a Liquid Chromatography-Mass Spectrometry/Mass Spectrometry instrument. The result is that the PSB extract showed its potential as an immunomodulatory and anticancer agent. The toxicity evaluation showed that the PSB extract was classified as a toxic material. More importantly, the PSB extract showed an activity against MCF-7 cancer with lethal concentration 50% (LC 50 ) values of 68.46 µg/mL and an inhibition concentration 50% (IC 50 ) of 362.36 µg/mL, respectively. The chemical evaluation suggested that the terpenoid and polyphenolic compounds are responsible for the immunomodulatory and anticancer mechanisms. In summary, our work showed that the PSB extract is a potential alternative source for the treatment of breast cancer and supports the immune response.
Prolonged use of nonsteroidal anti-inflammatory drugs (NSAIDs) can trigger ulcers in the gastric mucosa. Pepolo stem bark (Bischofia javanica Blume) has been empirically used to treat gastric ulcers. This plant contains alkaloids, flavonoids, tannins, saponins, and triterpenoids with gastroprotective potential. This study aims to determine the gastroprotective effect of pepolo stem bark extract (PSBE) against gastric ulcers of Wistar rat induced by Aspirin. Aspirin (150 mg/kg BW) was administered orally for eight days to all the groups except normal control. After 4 h of induction, normal and negative control received Na CMC 0.5%, while the positive group received Omeprazole (3.6 mg/kg BW). The remaining low, middle, and high dose groups received PSBE (100, 200, and 300 mg/kg BW). On the 9 th day, the rats were dissected and tested on the stomach organs. The parameters were scored based on the severity of rat peptic ulcers, ulceration index, and percentage protection ratio. The scoring data were analyzed using the Kruskal-Wallis non-parametric test and post hoc the Mann-Whitney test. The administration of PSBE showed the ability to reduce the ulcer index and increase the percentage of ulcer inhibition significantly compared to the negative control (p<0.05), thus showed that PSBE could be a promising gastroprotective herbal medicine.
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