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Histone methylation is thought to control the regulation of genetic program and the dysregulation of it has been found to be closely associated with cancer. JMJD3 has been identified as an H3K27 demethylase and its role in cancer development is context specific. The role of JMJD3 in gastric cancer (GC) has not been examined. In this study, JMJD3 expression was determined. The prognostic significance of JMJD3 and its association with clinical parameters were evaluated. JMJD3 dysregulation mechanism and targets were analyzed. The effect of JMJD3 mutation was determined by functional study. Results showed that JMJD3 was overexpressed in different patient cohorts and also by bioinformatics analysis. High JMJD3 expression was correlated with shortened overall survival in patients with GC and was an independent prognosis predictor. Genetic aberration and DNA methylation might be involved in the deregulation of JMJD3 in GC. Downstream network of JMJD3 was analyzed and several novel potential targets were identified. Furthermore, functional study discovered that both demethylase-dependent and demethylase-independent mechanisms were involved in the oncogenic role of JMJD3 in GC. Importantly, histone demethylase inhibitor GSK-J4 could reverse the oncogenic effect of JMJD3 overexpression. In conclusion, our study report the oncogenic role of JMJD3 in GC for the first time. JMJD3 might serve as an important epigenetic therapeutic target and/or prognostic predictor in GC.

High molecular weight poly (lactic acid) (PLA) was obtained by chain extending with hexamethylene diisocyanate (HDI). The influences of the amount of chain extender, reaction time, and molecular weight changes of prepolymers on the poly(lactic acid) were investigated. PLA prepolymer with a viscosity, average molecular weight (Mη) of 2 × 104 g/mol was synthesized froml-lactide using stannous octoate as the catalyst. After 20 min of chain extension at 175°C, the resulting polymer hadMwof 20.3 × 104 g/mol andMnof 10.5 × 104 g/mol. Both FT-IR and1H-NMR verified that the structure of PLA did not change either before chain extending or after. The optically active characterized that the chain extending-product was left handed. DSC and XRD results showed that both theTgand the crystallinity of PLA were lowered by chain-extension reaction. The crystalline transformation happened in PLA after chain extending, crystallineα′form toαform.

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Prophylactic administration of NSAIDs reduced the incidence of PEP in both RCTs and C-Cs, especially when rectally administered, but was not effective in reducing the risk of moderate to severe pancreatitis.

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The types of internal fault and their influencing factors in GIS are analyzed. The key technologies and researching results of UHF, ultrasound and SF6 decomposition method on site for GIS internal fault detecting are presented, compared with each other and analyzed. The methods of fault location and the fault type classification method are summarized finally.