In this Feature, electrochemiluminescent (ECL) and photoelectrochemical (PEC) properties and mechanisms of semiconductor quantum dots (QDs) are reviewed, with emphasis on their specific fundamentals and concise comparison on their similarities and differences. With recent illustrative examples of bioanalytical applications, the main signaling strategies for QDs-based ECL and PEC bioanalysis are then highlighted. The future prospects in this field are also discussed.

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This work reports a plasmonic strategy capable of label-free yet amplified photoelectrochemical (PEC) immunoassay for the sensitive and specific detection of model protein p53, an important transcription factor that regulates the cell cycle and functions as a tumor suppressor. Specifically, on the basis of Au nanoparticles (NPs) deposited on hierarchically ordered TiO2 nanotubes (NTs), a protein G molecular membrane was used for immobilization of alkaline phosphatase (ALP) conjugated anti-p53 (ALP-a-p53). Due to the immunological recognition between the receptor and target, the plasmonic charge separation from Au NPs to the conduction band of TiO2 NTs could be influenced greatly that originated from multiple factors. The degree of signal suppression is directly associated with the target concentration, so by monitoring the changes of the plasmonic photocurrent responding after the specific binding, a new plasmonic PEC immunoassay could be tailored for label-free and amplified detection. The operating principle of this study could be extended as a general protocol for numerous other targets of interest.

Photoelectrochemical (PEC) immunoassay is an attractive methodology as it allows for an elegant and sensitive protein assay. However, advanced PEC immunoassay remains challenging and the established amplifications rely almost exclusively on the labeling of various enzymes, which usually suffer the inferior stabilities. Here we report the development and validation of the DNA labeling that leads to a unique amplification probe for the sensitive PEC immunoassay of HIV-1 capsid protein, p24 antigen, an important biomarker of human immune deficiency virus (HIV). Following the sandwich immunobinding, the DNA tags could be released and the subsequent dipurinization of the oligonucleotide strands enables the easy oxidation of free nucleobases at a CdTe quantum dots (QDs) modified ITO transducer. Such DNA tags induced PEC amplification and readout permits the exquisite assay of HIV-1 p24 antigen with high sensitivity. As compared to the existing method of enzymatic labeling, the easy preparation and stability of these labels make them very suitable for PEC amplification. Another merit of this method is that it separates the immunobinding from the PEC transducer, which eliminates the commonly existing affection during the biorecognition processes. This work paves a new route for the PEC immunoassay of HIV-1 p24 antigen and provides a general format for the PEC biomolecular detection by means of the DNA labeling.

This work reports the innovative design and application of a three-dimensional (3D) TiO 2 @Cu 2 O@nickel foam electrode synergized with enzyme catalysis toward the proof-of-concept study for oxygen-independent photocathodic enzymatic detection. Specifically, a 3D-nanostructured photoelectrode has great potential in the semiconductor-based photoelectrochemical (PEC) biological analysis. On the other hand, using various photocathodes, cathodic PEC bioanalysis, especially the photocathodic enzymatic detection, represents an attractive frontier in the field. Different from state-of-the-art photocathodic enzymatic studies that are oxygen-dependent, herein, we present the ingenious design, characterization, and implementation of 3D TiO 2 @ Cu 2 O@nickel foam photocathodes for the first oxygen-independent example. In such a configuration, the Cu 2 O acted as the visible-light absorber, while the TiO 2 shell would simultaneously function as a protective layer for Cu 2 O and as a desirable substrate for the immobilization of enzyme biomolecules. Especially, because of the proper band positions, the as-designed photocathode exhibited unique O 2 -independent PEC property. Exemplified by glucose oxidases, the as-developed sensor exhibited positive response to glucose with good performance. Because various oxidases could be integrated with the system, this protocol could serve as a universal O 2 -independent platform for many other targets. This work is also anticipated to catalyze more studies in the advanced 3D photoelectrodes toward innovative enzymatic applications.

We have developed sensitive photoelectrochemical (PEC) detection of cysteine using the gold nanoparticles (Au NPs) equipped perovskite BiNbOCl heterostructure. The BiNbOCl was prepared by a solid-state reaction, and the Au NPs/BiNbOCl electrode was made through the electrostatic layer-by-layer self-assembly technique. The Au NPs/BiNbOCl electrode provided much enhanced photocurrent with a great increase compared to the bare BiNbOCl electrode and allowed for the plasmon-enhanced PEC detection of cysteine with good performance. It demonstrated rapid response, high stability, wide linear detection range and certain selectivity, implying its great promise in its application. Therefore, the Au NPs/BiNbOCl heterostructure has provided a promising platform for the development of PEC bioanalysis. More generally, these findings offered an insight into the exploitation of perovskite materials for PEC bioanalytical purposes.

This work reports the first synthesis and characterization of a ferroelectric perovskite oxide-based heterostructure as well as its application for photoelectrochemical (PEC) bioanalytical purposes. Specifically, exemplified by [KNbO][BaNiNbO] (KBNNO), the ferroelectric perovskite oxides were prepared by solid-state synthesis, while the TiO nanorod (NR) arrays were obtained via a hydrothermal method. Using the technique of pulsed laser deposition (PLD), KBNNO were then deposited on TiO NRs to form KBNNO@TiO NR heterostructures. Various characterization techniques were applied to reveal compositional and structural information on the as-fabricated sample, and favorable alignment existed between the two components as displayed by the PEC test. In the detection of l-cysteine, the as-fabricated KBNNO@TiO NRs demonstrated good performance in terms of sensitivity and selectivity. This work revealed the potential of ferroelectric perovskite oxide and its heterostructures for innovative PEC bioanalytical applications, and we hope it will generate more interest in the development of various ferroelectrics-based heterostructures for advanced PEC bioanalysis.

An alkylthieno-2-yl flanked dithieno[2,3-d:2 0 ,3 0 -d 0 ]benzo[1,2-b:4,5-b 0 ]dithiophene-based low band gap conjugated polymer for high performance photovoltaic solar cells † A low band gap conjugated polymer derived from alkylthieno-2-yl flanked dithieno[2,3-d:2 0 ,3 0 -d 0 ]benzo [1,2-b:4,5-b 0 ]dithiophene and a naphtho[1,2-c:5,6-c]bis[1,2,5]thiadiazole derivative was first synthesized, and high performance inverted photovoltaic solar cells with a power conversion efficiency of 7.52% have been demonstrated.