Cathodic photoelectrochemical bioanalysis

Xu, Yi‐Tong; Yu, Si‐Yuan; Zhu, Yuan‐Cheng; Fan, Gao-Chao; Han, Deman; Qu, Peng; Zhao, Weiwei

doi:10.1016/j.trac.2019.03.002

“…[9] The p-type NiO [10] was chosen for the construction of photocathodic nanotool due to its good accessibility, high stability, and excellent anti-interference capability from reductive substances in biological samples. [11] Meanwhile, a representing benzopyrylium-coumarin-structured organic probe (denoted as BCOP) [12] was synthesized and then anchored onto the NiO film. [13] Significantly, the BCOP could serve simultaneously as the target-specific organic probe and the efficient sensitizer for NiO.…”

mentioning

confidence: 99%

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

Ruan

¹

,

Chen

²

,

Xu

³

et al. 2021

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With reduced background and high sensitivity, photoelectrochemistry (PEC) may be applied as an intracellular nanotool and open a new technological direction of single‐cell study. Nevertheless, the present palette of single‐cell tools lacks such a PEC‐oriented solution. Here a dual‐functional photocathodic single‐cell nanotool capable of direct electroosmotic intracellular drug delivery and evaluation of oxidative stress is devised by engineering a target‐specific organic molecule/NiO/Ni film at the tip of a nanopipette. Specifically, the organic molecule probe serves simultaneously as the biorecognition element and sensitizer to synergize with p‐type NiO. Upon intracellular delivery at picoliter level, the oxidative stress effect will cause structural change of the organic probe, switching its optical absorption and altering the cathodic response. This work has revealed the potential of PEC single‐cell nanotool and extended the boundary of current single‐cell electroanalysis.

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“…Sequential Ni deposition via magnetron sputtering and annealing was conducted to in situ form NiO/Ni film as the semiconducting and metal contact layers, followed by a wax sealing step to expose a specific working area [9] . The p‐type NiO [10] was chosen for the construction of photocathodic nanotool due to its good accessibility, high stability, and excellent anti‐interference capability from reductive substances in biological samples [11] . Meanwhile, a representing benzopyrylium‐coumarin‐structured organic probe (denoted as BCOP) [12] was synthesized and then anchored onto the NiO film [13] .…”

Section: Figurementioning

confidence: 99%

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

Ruan

¹

,

Chen

²

,

Xu

³

et al. 2021

View full text Add to dashboard Cite

With reduced background and high sensitivity, photoelectrochemistry (PEC) may be applied as an intracellular nanotool and open a new technological direction of single‐cell study. Nevertheless, the present palette of single‐cell tools lacks such a PEC‐oriented solution. Here a dual‐functional photocathodic single‐cell nanotool capable of direct electroosmotic intracellular drug delivery and evaluation of oxidative stress is devised by engineering a target‐specific organic molecule/NiO/Ni film at the tip of a nanopipette. Specifically, the organic molecule probe serves simultaneously as the biorecognition element and sensitizer to synergize with p‐type NiO. Upon intracellular delivery at picoliter level, the oxidative stress effect will cause structural change of the organic probe, switching its optical absorption and altering the cathodic response. This work has revealed the potential of PEC single‐cell nanotool and extended the boundary of current single‐cell electroanalysis.

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“…Photogenerated electrons are prone to reacting with electron acceptors but inert to those easily oxidizable interferents. [20][21][22][23] Therefore, developing PEC bioassay systems based on the principle of photocathodic measurement of enzymatic product H 2 O 2 would improve the selectivity. However, practical applications of the photocathodic measurement principle have been restricted by concomitant oxygen reduction reactions at similar potentials.…”

Section: Introductionmentioning

confidence: 99%

High-Performance Photocathodic Bioanalysis Based on Core–Shell Structured Cu ₂ O@TiO ₂ Nanowire Arrays with Air–Liquid–Solid Joint Interfaces

Wang

¹

,

Chen

²

,

Wang

³

et al. 2022

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Developing photoelectrochemical (PEC) bioassays based on the principle of a photocathodic measurement of enzymatic product H 2 O 2 is highly attractive because it can naturally avoid interfering signals arising from reductive species inherent to biofluids. However, fluctuant oxygen levels in the analyte solution can compromise the accuracy of photocathodic bioanalysis and restrict its application because oxygen reduction potential is similar to H 2 O 2 . Herein, we addressed this restriction by constructing a triphase biophotocathode with air-liquid-solid joint interfaces by immobilizing an oxidase enzyme film on the tip part of superhydrophobic p-type semiconductor nanowire arrays. Such a triphase biophotocathode has a reaction zone with steady and air phasedependent oxygen concentration which stabilizes and increases the oxidase kinetics, and enables the photocathodic measurement principle in reliable PEC bioassay development with high selectivity, good accuracy, and a wide linear detection range. Moreover, the biophotocathode shows good stability during repeated testing under light illumination. This reliable PEC bioassay system has broad potential in the fields of disease diagnosis, medical research, and environmental monitoring.

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Cathodic photoelectrochemical bioanalysis

Cited by 111 publications

References 76 publications

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

High-Performance Photocathodic Bioanalysis Based on Core–Shell Structured Cu ₂ O@TiO ₂ Nanowire Arrays with Air–Liquid–Solid Joint Interfaces

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Cathodic photoelectrochemical bioanalysis

Cited by 111 publications

References 76 publications

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

High-Performance Photocathodic Bioanalysis Based on Core–Shell Structured Cu 2 O@TiO 2 Nanowire Arrays with Air–Liquid–Solid Joint Interfaces

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High-Performance Photocathodic Bioanalysis Based on Core–Shell Structured Cu ₂ O@TiO ₂ Nanowire Arrays with Air–Liquid–Solid Joint Interfaces