Aim. To determine the prognostic value of lactate concentration in amniotic fluid and umbilical cord blood of newborns at early neonatal period as indicators of increased risk of unfavorable outcome in infants with perinatal hypoxia. Methods. Determination of lactate and creatinine concentration in amniotic fluid at first stage of labor was performed by biochemical means. Determination of lactate concentration in the blood serum of infants at birth and at 3-4 days of life was also performed. Results. In timely delivery associated with perinatal hypoxia lactate concentration in amniotic fluid was increased compared to the lactate concentration in amniotic fluid during normal labor (10.6±3.18 vs 7.1±1.45 mmol/l, p 0.001). Recalculation of the lactate concentrations in amniotic fluid considering its dilution has showed a substantial difference in concentrations (0.083±0.016 vs. 0.039±0.003 mmol/l of lactate per 1 mmol/l creatinine, p 0.001). Cord blood serum lactate concentrations in newborns with perinatal hypoxia was higher compared to healthy infants (16.4±4.96 vs 9.5±3.16 mmol/l, p 0.001). Lactate concentration in umbilical cord blood serum in early neonatal period newborns rose (p 0.001), and in infants with perinatal hypoxia the increase was more significant compared to in healthy newborns (28.1±7.75 vs 13.3±3.50 mmol/l, p 0.001). Conclusion. The determination of lactate concentration in amniotic fluid at first stage of labor is an effective method to diagnose perinatal hypoxia. In early neonatal period an increase of lactate concentration in newborns, especially in newborns with perinatal hypoxia, was found.

The aim of this study was to determine reference values of matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in the amniotic fluid at the first stage of labor in physiological pregnancy. 89 women at the first stage of term labor have been examined. Samples of amniotic fluid were taken at the first period of labor by vaginal amniotomy. Concentrations ofMMP-1, MMP-2, MMP-9, and TIMP-1 were investigated in amniotic fluid by ELISA kits. We have determined normal concentration ranges for MMP-1, MMP-2, MMP-9, TIMP-1, and ratios of concentrations of MMPs and TIMP-1 (MMP-1/TIMP-1, MMP-2/TIMP-1, MMP-9/TIMP-1) in the amniotic fluid at the first period of labor in physiological pregnancy. These included: MMP-1 - 5.1-16.8 pg/mg of protein, MMP-2 - 238.3-374.1 pg/mg of protein, MMP-9 - 66.1-113.3 pg/mg of protein, TIMP-1 - 4.7-13.6 pg/mg of protein, ratio of MMP-1/TIMP-1 - 0.1-2.2, ratio of MMP-2/TIMP-1 - 19.9-55.7, ratio of MMP-9/TIMP-1 - 4.2-17.2.

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Учредитель и издатель Федеральное государственное бюджетное образовательное учреждение высшего образования «Алтайский государственный медицинский университет» Министерства здравоохранения Российской Федерации (ФГБОУ ВО АГМУ Минздрава России) 656038, РФ, Алтайский край, Барнаул, проспект Ленина, 40 www.asmu.ru РЕДАКЦИЯ Главный редактор Салдан Игорь Петрович доктор медицинских наук, профессор Заместитель главного редактора Жариков Александр Юрьевич доктор биологических наук, доцент Редактор-организатор Киселев Валерий Иванович член-корреспондент РАН, доктор медицинских наук, профессор Выпускающий редактор Широкоступ Сергей Васильевич кандидат медицинских наук, доцент Научные редакторы Брюханов Валерий Михайлович доктор медицинских наук, профессор Колядо Владимир Борисович доктор медицинских наук, профессор Лукьяненко Наталья Валентиновна доктор медицинских наук, профессор Шойхет Яков Нахманович член-корреспондент РАН, доктор медицинских наук, профессор Редакционная коллегия Брико Николай Иванович академик РАН, доктор медицинских наук, профессор Воевода Михаил Иванович академик РАН, доктор медицинских наук, профессор Дыгай Александр Михайлович академик РАН, доктор медицинских наук, профессор Злобин Владимир Игоревич академик РАН, доктор медицинских наук, профессор Лобзин Юрий Владимирович академик РАН, доктор медицинских наук, профессор Мадонов Павел Геннадьевич доктор медицинских наук, профессор Онищенко Геннадий Григорьевич академик РАН, доктор медицинских наук, профессор Павлова Наталья Григорьевна доктор медицинских наук, профессор Полушин Юрий Сергеевич академик РАН, доктор медицинских наук, профессор Рахманин Юрий Анатольевич академик РАН, доктор медицинских наук, профессор Ответственный за перевод Широкова Валерия Олеговна БЮЛЛЕТЕНЬ МЕДИЦИНСКОЙ НАУКИ №3 (11) 2018 2 СОДЕРЖАНИЕ Науки о здоровье Влияние минеральной воды на течение экспериментальной гастропатии Онищенко Г.Г.

Background: The research objective was to study the effect of the tetrapeptide Leu-Ile-Lys-His on the course of experimental oxalate nephrolithiasis. Methods and Results: Experiments were conducted on 30 male Wistar rats, which were divided equally into 3 groups: the control group (6 weeks of ON modeling), the test group (6 weeks of ON modeling+the tetrapeptide Leu-Ile-Lys-His introduction at a dose of 12 mg/kg), and the experimental group (determination of indicators of free radical oxidation process activity in intact rats). Every 7 days, indicators of the excretory renal function (the level of diuresis and creatinine excretion) and activity of marker enzymes of renal tissue damage (lactate dehydrogenase and γ-glutamyl transferase) were determined in the urine of rats. After six weeks, we determined parameters of activity of the free radical oxidation process in the kidneys of rats posthumously and conducted the morphological study for formation of calcium deposits. Conclusion: Experiments showed that in the control group there were characteristic signs of nephrolithiasis development: increase of LDH activity by 5.4 times, increase of free radical oxidation process activity in the kidneys, and formation of large concrements in the kidneys, the mean size of which was 298.8±34.2 μm 2. In the test group, there was weakening of LDH and GGT activity and weakening of free radical oxidation process activity. There were no morphological signs of nephrolithiasis development in the kidneys.

The research objective was to investigate the pathological picture of diabetic nephropathy in the 8-month streptozotocin (STZ)-induced diabetes mellitus and compare it with the previously obtained picture of diabetic nephropathy in the 1-month STZinduced diabetes mellitus (DM). Materials and methods: Experiments were conducted on 15 male Wistar rats aged 2-3 months and weighing 250-280 g. To induce DM, the animals were injected intraperitoneally 1ml of STZ solution in the citrate buffer at a dose of 65 mg/kg. In accordance with modern ideas about the peculiarities of DM modeling using STZ for more selective modeling of type 2 DM, the rats were previously injected with an intraperitoneal solution of cytoflavin based on a nicotinamide dose of 115 mg/kg. After 8 months of the experiment, the concentration of glucose, protein, and creatinine was determined in urine. The animals were euthanized under ethereal anesthesia and both kidneys were extracted, cleaned and washed with a physiological solution. In the renal tissues of animals, we determined the concentration of thiobarbituric acid reactive products, total pro-oxidant activity, total antioxidant activity, activity of antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase). With the help of a morphometric method of research, the area of renal glomeruli and the area of capillary lumens were measured, and after a special computer processing of digital photos, the total area of the vascular bed in the glomerulus and the area of mesangium in the renal glomerulus were assessed. Results: Diabetic nephropathy in the 8-month STZ-induced DM was more pronounced than with the 1-month STZ-induced DM. The revealed biochemical and morphological signs of the 8-month STZ-induced DM indicate the irreversible nature of its course and the impossibility of its pharmacological correction.

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