Hypoxia is a typical pathological process developing due to the decreased concentration of oxygen in the organism or in the particular tissues and organs, as well as disturbances of oxygen utilization during biological oxidation. Aim. To determine the antihypoxic and antioxidant activity of the ZNM derivative of 2-benzamido-2-(2-oxoindolin-3-iliden) acetic acid in the brain and blood plasma of rats under the conditions of chronic hypobaric hypoxia (CHH) by the indicators of the lipid peroxidation processes activity and the state of the antioxidant defense. Materials and methods. The studies were conducted under the conditions of CHH modeled by the standard method. The ZNM substance in the dose of 15 mg/kg and the reference drug mexidol in the dose of 100 mg/kg were injected intraperitoneally starting from the 14th day of the experiment 30 min prior to the hypoxia simulation. Results. In the group of animals treated with ZNM a significant decrease in the content of malondialdehyde in the blood plasma and brain structures (by 1.2 and 1.1 times, respectively), as well as in the content of oxidative modified proteins (by 1.2 times in the blood plasma and 1.4 times in the brain tissue) was observed. The content of ceruloplasmin in the blood plasma decreased by 1.7 times; the content of SH-groups increased by 3.1 times; the catalase activity increased by 1.2 times in the blood plasma, and by 1.9 times in brain homogenates. The reduced glutathione level in brain homogenates increased by 1.4 times; the glutathione peroxidase activity significantly increased by 2.1 times in the blood plasma and by 1.3 times in brain homogenates compared to the model pathology group. Conclusions. It has been found that both mexidol and the ZNM substance demonstrate a significant antioxidant activity under the conditions of CHH by stabilizing the balance between prooxidant and antioxidant systems of the body in rats, and it is particularly important in oxidative stress caused by chronic hypoxia. Key words: antihypoxants; chronic hypobaric hypoxia; 2-benzamido-2-(2-oxoindolin-3-iliden) acetic acid derivative; mexidol І. І. Заморський, Ю. С. Букатару, С. В. Колісник* Вищий державний навчальний заклад України «Буковинський державний медичний університет» Національний фармацевтичний університет* Вплив похідного 2-бензамідо-2-(2-оксоіндолін-3-іліден) оцтової кислоти на активність процесів ліпопероксидації та стан антиоксидантного захисту при хронічній гіпобаричній гіпоксії Гіпоксія -типовий патологічний процес, що виникає при зниженому вмісті кисню в організмі або окремих тканинах і органах, а також при порушенні утилізації кисню в процесі біологічного окиснення. Мета. Метою дослідження стало встановлення антигіпоксантної та антиоксидантної активності похідного 2-бензамідо-2(2-оксоіндолін-3-іліден) оцтової кислоти ZNM в головному мозку та плазмі крові щурів за умов хронічної гіпобаричної гіпоксії (ХГГ) за показниками активності процесів ліпопероксидації та стану антиокси-дантного захисту у щурів. Матеріали та методи. Дослідження проводились за ...
In experiments on adult laboratory rats the effect of the derivative of 2-benzamido-2-(2-oxoindolin-3-iliden) acetic acid under the symbol ZNM on the activity of the lipid and protein peroxidation and antioxidant system in blood plasma and brain, as well as on the activity of the key enzyme of energy metabolism succinate dehydrogenase in the brain of animals with a closed traumatic brain injury (TBI) of moderate severity in comparison with the reference drug mexidol was investigated. It was established that ZNM not significantly concedes to the action of mexidol in its cerebroprotective on antioxidant properties under the conditions of TBI, contributing to normalization of the prooxidantantioxidant balance in the blood plasma and brain of rats, as well as improving the energy metabolism in the cells of the central nervous system.
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