ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Based on the notions of supramolecular nonenzymatic interaction of amyloid with known fluorescent probes (thioflavin T and Congo red) a search for the substances, which may be perspective as fluorescent probes selective to amyloid in the line of 4-N-aryl-3,5-dioxo-1-formyl-10-oxa-4-azatricyclo[5.2.11.7.02.6]dec-8-ene derivatives has been undertaken.Dry crystals of the chosen substances having the structure of the molecular rotor similar to thioflavin T emitted light in the visible range of 421.0-435.5 nm, when excited by ultraviolet. Dissolution of the substances in 96° ethanol extinguishes fluorescence. wateralcohol solutions of these substances (0.75%; water:ethanol 96°=1:1, pH>7) also did not emitted the light (beyond the sensitivity of photomultiplier FEU-39). Treating with alcohol solutions of the tested preparations the depersonalized deparaffinated, preliminarily stained with hematoxylin, human kidney sections with clinically and histologically proved kidney amyloidosis was not accompanied by a marked fluorescence. Staining of the sections of the same kidney with water-alcohol alkalized solution of these preparations after imbedding into the non-fluorescent transparent medium was accompanied by luminescence of all kidney structures in the visible range at a wavelength of 534 nm with a more intensive luminescence of amyloid deposits than cellular structures.Conclusion. The result obtained opens good perspectives of using the tested substances as novel fluorophores for detecting amyloid.
Amino‐containing derivatives of various structures have been obtained via the Mannich reaction in a series of bicyclic phosphonates based on 2‐methylresorcinol, resorcinol, and pyrogallol with paraformaldehyde and secondary amines via the Mannich reaction. The structure and composition of the obtained compounds have been determined using 31Р and 1Н NMR spectroscopy, IR spectroscopy, mass spectrometry (MALDI‐TOF), and X‐ray analysis.
We have previously shown that the reaction of bicyclo[4.1.0]heptane-7-carbonyl chloride with medium esters of phosphorous and arylphosphonous acids occurs with the retention of the small carbocycle [1]. In continuation of this work it is interesting to study the behavior of carbonyl compounds of spirohexane series in the Arbuzov reaction [2]. Due to the high energy strain of these spiranes one could expect unusual reaction course affording new types of organophosphorus compounds.We found that spiro[2.3]hexane-5-carbonyl chloride I reacts readily with phosphorous and arylphosphonous acids esters. When an equimolar ratio of reagents was used under mild conditions (0-20°C), the attack of the P-nucleophiles occurs on the carbon atom of the carbonyl group. As a result, the reaction produces dialkyl spiro[2.3]hexane-5-carbonyl phosphonates IIa-IIc or alkylarylspiro[2.3]hexane-5-carbonyl phosphinates IId-IIf.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.