Novel Fluorescent Probes for Amyloid Detection

Сапожников, С. П.; Karyshev, P. B.; Шептухина, А И; Nikolayeva, O. V.; Avruyskaya, A. A.; Mitrasov, Yu. N.; Kozlov, Vadim A.

doi:10.17691/stm2017.9.2.11

Cited by 6 publications

(1 citation statement)

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“…One of the approaches to improve amyloid detection is to apply analogues of Congo red and thioflavin T [ 13 ]. It has been shown by us that one of the fluorene-based analogues of Congo red, disodium salt of 2,7-(1-amino-4sulfo-2naphthylazo)fluorine (DSNAF) can be successfully applied to identify amyloid deposits in the tissues of laboratory animals [ 14 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Amyloid Detection in Human Tissue Load Using a Fluorescent Dye — Congo Red Analogue

Гусельникова¹,

Antimonova²,

Федорова³

et al. 2020

Sovrem Tehnol Med

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The aim of the study was to develop a new technology for the detection of amyloid in human tissues based on the fluorescent dye, disodium salt of 2,7-(1-amino-4-sulfo-2-naphthylazo)fluorene (DSNAF). Materials and Methods. Synthesis of DSNAF was performed by diazotization of 2,7-diaminofluorene in a stream of argon followed by azo coupling with naphthionic acid. Identification of DSNAF was performed using MALDI mass spectrometry. Human myocardial samples from males and females aged from 85 to 98 years (n=11) were the material for the histochemical study. Myocardial paraffin sections were stained with a 0.1% aqueous solution of Congo red or with an aqueous solution (0.1 or 0.034%) of DSNAF under the same conditions. Results. It has been demonstrated for the first time that a new fluorene-based analogue of Congo red, DSNAF, can be successfully used to identify amyloid deposits in histological sections of human myocardium. In terms of the specificity and intensity of amyloid staining, DSNAF is comparable to Congo red, which is the gold standard for detecting amyloid deposits. The fluorescence intensity of DSNAF when binding to amyloid fibrils is significantly higher than the intensity of Congo red fluorescence (with a lower intensity of background fluorescence of heart muscle tissue). This is especially useful for identifying small deposits of amyloid in the human tissues which is important when using small biopsies. Conclusion. The advantages of using DSNAF allow us to consider the developed technology for the detection of amyloid as a new promising method of identifying amyloid deposits in human tissues.

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Section: Introductionmentioning

confidence: 99%