Multiplex real-time PCR with quantification of targets in a single fluorescence channel has been the demand in biotechnology industry. Here, we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this technique, we show the intensity of the fluorescence signals of two discrete Tm targets is different at certain temperatures called detection temperatures, by which a high Tm target can be detected regardless of a low Tm target. We then identify the low Tm target by utilizing a change of the fluorescence signals between two different detection temperatures. Furthermore, it enables us to determine quantification of each target in a single channel, possibly facilitating convenient patient care for drug treatment in clinics.
A blind equalization algorithm may be switched to a decision-directed(DD) scheme in order to speed up the convergence rate. In this paper, we propose a new blind equalization scheme that automatically switches to the DD mode. The proposed scheme operates in two mode: blind equalization mode and a DD mode. The mode is determined by the region in which the equalizer output lies, and the region varies as a function of the estimate value of the error variance. Only the equalized signal that falls in the region of confidence is used to calculate the variance. From simulation results, it is shown that this scheme can achieve faster convergence and small MSE.
This study attempted to investigate relations among self-management, job commitment, hairstyling continuance intention and enjoyment factors and empirically analyze the mediating effects of enjoyment factors. For this, a questionnaire survey was performed against hairdressers in Seoul, Gyeonggi-do and Incheon in 2022, and a total of 331 copies were used for final analysis. The collected data were analyzed by descriptive statistics, validity analysis, reliability analysis and correlation analysis, using SPSS 26.0. In addition, confirmatory factor analysis, structural equation model validation and bootstrapping were conducted, using AMOS 26.0. The results found that Self-management positive influence enjoyment and job commitment, and enjoyment positively affected job commitment and hairstyling continuance intention. In addition, delight showed positive mediating effects in the influence of self-management on job commitment and hairstyling continuance intention. Therefore, a program should maintains proper self-management and positive enjoyment.
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