Objective: The objective of this research was to isolate and identify the major compound from the antioxidant extract obtained from the leaves of Bubonium graveolens.Methods: A bioguided fractionation was done to isolate and identify the major compound responsible of the antioxidant activity. The chemical structure of the isolated natural compound was established by spectroscopic means including UV, Fourier transform infrared, 1H NMR, and13C NMR. The purified compound was accurately tested for its antioxidant activity by the 2, 2-diphenyl-1-picrylhydrazyl assay at 517 nm.Results: The major compound named myricetin 3′-O-rhamnoside was isolated for the 1st time from the leaves of B. graveolens a medicinal specie of Algerian Sahara belonging to the Asteraceae family. The study showed that the myricetin 3ʹ-O-rhamnoside has an antioxidant potential 59% when compared with standard ascorbic acid with an IC 50=0.916 mg/ml.Conclusion: In the present work, it was possible to isolate and identify for the 1st time the major compound from the antioxidant fraction of B. graveolens.
This study aimed to examine the anti-termite potential of four Saharan plant extracts, namely, the apple of Sodom or rubber bush, Calotropis procera; pergularia, Pergularia tomentosa; jimsonweed, Datura stramonium, and Egyptian henbane, Hyoscyamus muticus from Bechar (southwest of Algeria) on workers of the harvester termite, Anacanthotermes ochraceus (Isoptera: Hodotermitidae). A direct contact application test was conducted with five fractions from aqueous extracts of each part of plant species (leaves, stems) using hexane, dichloromethane, ethyl acetate, butanol, and exhausted fraction. A repellent test was realized with aqueous extracts (10%) of plant species leaves and stems. According to the direct contact application test, all tested plants fractions showed termiticidal activities with different degrees. Butanolic fractions presented the best effects from leaves of C. procera and P. tomentosa with median lethal time (LT50) = 231.03 and 244.96 min, respectively. In the second test, wood samples were exposed to termite attack for four weeks, and the weight loss percentage was determined. The weight loss ranged from 0.034 to 16.90% at concentrations of 10% of plant extracts. The best repellent effect was obtained from leaves of C. procera (weight loss = 0.034%) and leaves of D. stramonium (weight loss = 1.29%). It was concluded that some Saharan plants are a good source of anti-termite compounds, especially C. procera.
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