Bioassay-Guided Isolation of the Major Compound With Antioxidant Activity From the Algerian Medicinal Plant Bubonium Graveolens

Abstract

Objective: The objective of this research was to isolate and identify the major compound from the antioxidant extract obtained from the leaves of Bubonium graveolens.Methods: A bioguided fractionation was done to isolate and identify the major compound responsible of the antioxidant activity. The chemical structure of the isolated natural compound was established by spectroscopic means including UV, Fourier transform infrared, 1H NMR, and13C NMR. The purified compound was accurately tested for its antioxidant activity by the 2, 2-diphenyl-1-picrylhydrazyl assay at 517 nm.Results: The major compound named myricetin 3′-O-rhamnoside was isolated for the 1st time from the leaves of B. graveolens a medicinal specie of Algerian Sahara belonging to the Asteraceae family. The study showed that the myricetin 3ʹ-O-rhamnoside has an antioxidant potential 59% when compared with standard ascorbic acid with an IC 50=0.916 mg/ml.Conclusion: In the present work, it was possible to isolate and identify for the 1st time the major compound from the antioxidant fraction of B. graveolens.

Comprehensive In Silico Screening of the Antiviral Potentialities of a New Humulene Glucoside from Asteriscus hierochunticus against SARS-CoV-2

“…Asteriscus hierochunticus (Michon) A. Wiklund is a widely distributed shrub that belongs to family Asteraceae and found in tropical regions, including Asia, the Americas, Africa, the Middle East and the Mediterranean region, Europe, and some islands in the Pacific and Atlantic oceans [24]. e genus Asteriscus is used in folk medicine for treatment and managing of different disease conditions, such as inflammations, fevers, cancers, stomach aches, bronchitis, hemorrhoids, and other disorders [25]. Phytochemical and pharmacological investigations on other members of the genus have been reported and resulted in the isolation of bioactive secondary metabolites such as sesquiterpenes, diterpenes, and phenolics [26], but for A. hierochunticus, there was no enough phytochemical investigation.…”

Analgesic and anti-inflammatory activity of aqueous extract of Bubonium graveolens

“…It is known locally as 'Tafss' 1 . Bubonium graveolens is used in the treatment of gastrointestinal disorders, fever, cephalic, dietary, and bronchitis, as well as an anti-inflammatory agent [5][6][7] . Recently it was used as a botanical fungicide 8,9 and was reported to possess antimicrobial and hypoglycemic activities 10,11 .…”