Stachydrine is a major constituent of Chinese herb leonurus heterophyllus sweet, which is used in clinics to promote blood circulation and dispel blood stasis. Our study aimed to investigate the role of stachydrine in human umbilical vein endothelial cells (HUVECs) injury induced by anoxia-reoxygenation. Cultured HUVECs were divided randomly into control group, anoxia-reoxygenation (A/R) group and 4 A/R+stachydrine groups. HUVECs in the control group were exposed to normoxia for 5 hours, while in all A/R groups, HUVECs underwent 3 hours anoxia followed by 2 hours reoxygenation, and HUVECs in the 4 A/R+stachydrine groups were treated with 10(-8) M, 10(-7) M, 10(-6) M and 10(-5) M (final concentration) of stachydrine respectively. After anoxia-reoxygenation, tissue factor (TF) was over-expressed, cell viability and the concentrations of SOD, GSH-PX and NO were declined, while LDH, MDA and ET-1 were over-produced (p < 0.05 to 0.001 vs. the control group). However, in stachydrine treated groups, TF expression was inhibited at both mRNA and protein levels, while the declined cell viability and SOD, GSH-PX, NO as well as the enhanced LDH, MDA and ET-1 levels occurred during anoxia-reoxygenation were ameliorated and reversed effectively (p < 0.05 to 0.01 versus A/R group). Consequently, our findings indicate that TF plays an important role in the development of anoxia-reoxygenation injury of HUVECs, stachydrine ameliorates HUVECs injury induced by anoxia-reoxygenation and its putative mechanisms are related to inhibition of TF expression.

Factor VIII (FVIII) is an essential component in blood coagulation, a deficiency of which causes the serious bleeding disorder hemophilia A. Recently, with the development of purification level and recombinant techniques, protein replacement treatment to hemophiliacs is relatively safe and can prolong their life expectancy. However, because of the possibility of unknown contaminants in plasma-derived FVIII and recombinant FVIII, and high cost for hemophiliacs to use these products, gene therapy for hemophilia A is an attractive alternative to protein replacement therapy. Thus far, the adeno-associated virus (AAV) is a promising vector for gene therapy. Further improvement of the virus for clinical application depends on better understanding of the molecular structure and fate of the vector genome. It is likely that hemophilia will be the first genetic disease to be cured by somatic cell gene therapy.

No abstract

Vestibular migraine (VM) is one of the most debilitating chronic diseases that is currently underdiagnosed and undertreated. The treatment of VM is a dynamic and rapidly advancing area of research. New developments in this field have the potential to improve the diagnosis and provide more individualized treatments for this condition. In this review, we discussed the progress of evidence-based treatment of VM, including pharmacotherapy and nonmedical methods. A search of the literature was conducted up to September 2019. In order to control or cure VM, patients should follow three steps. First, patients should comply with diet and behavioral medication; Second, during the attack of VM, patients should take medicine to control the symptoms. These acute attack treatment of VM consists of antiemetic medications (e.g., dimenhydrinate and benzodiazepines), anti-vertigo medicine, and analgesics (e.g. triptans). Third, prophylactic medicine (e.g., propranolol, topiramate, valproic aid, lamotrigine, and flunarizine) can be used to reduce the frequency and severity of VM attack. Also, vestibular rehabilitation (VR) treatment should be considered for all VM. Meanwhile, we also propose to establish a culture of prevention which is essential for reducing the personal, social and economic burden of VM.

In order to solve network security problem, we need identity authentication protocol to ensure legal user's authority. Multi-factor identity authentication protocols have more merits than the common identity authentication protocol. This paper analyzes the shortcomings of existing network identity authentication methods, and proposes a new authentication protocol based on S M2 and fingerprint US Bkey. The proposed scheme,which Combines the fingerprint US Bkey of fingerprint certificate with the National cipher algorithm S M2, constructs the multi-factor authentication model. And the scheme obtains many merits:1) it adopts the challenge response authentication mechanism, and realizes the multi-factor mutual authentication.2) it implements US Bkey to verify the user and the remote server authentication by fingerprint features. 3) it can prevent eavesdropping attack, impersonation attack, replay attack and dos attack effectively. 4) it has better calculation and security performance than the existed schemes.

Weidean, a formulation of Chinese medicinal herbs and other constituents, was associated with AIHA in a woman taking the remedy for gastritis.

Background Dizziness is a common and challenging condition among population. There is little published study which surveys the characteristics of dizziness of inpatients in the department of neurology. Objective This study was to investigate the inpatients with dizziness as the chief complaint in a neurology department. Materials and Methods We conducted a retrospective study of inpatients with dizziness attending a tertiary neurological department in Beijing. We audited 211 patients with dizziness as the chief complaint from 1841 patients discharged from our tertiary neurological department. Results The inpatients with dizziness as the chief complaint accounted for 11.5% of all inpatients. Dizziness was more common in women than in men (p=0.004). There were more patients presenting with vertigo (40.8%) and light-headedness (39.8%) than disequilibrium (17.1%) and pre-syncope (2.4%). Nausea (48.3%), vomiting (34.1%), headache (13.3%), walk unsteadily (13.3%) and ear symptoms (12.8%) were the most common accompanying symptoms. Hypertension, diabetes, cerebrovascular diseases, dyslipidemia, and coronary heart disease were the most common diseases in past medical history. Dix-Hallpike test (24.6%) and Romberg’s sign (11.4%) were positive in dizziness patients. Nystagmus (2.4%), vision changes (1.4%) and hearing disorders (8.5%) were relatively rare symptoms. MRI (60.2%), CT (31.8%), carotid duplex ultrasound (30.8%), echocardiography (28.0%) were common auxiliary examination. Benign paroxysmal positional vertigo (24.2%) and stroke/transient ischemic attack (19.0%) were common causes of dizziness. 97.2% of inpatients with dizziness can be improving after treatment. Conclusion Dizziness was a common and challenging condition. Vertigo and light-headedness were the most common dizziness types. Benign paroxysmal positional vertigo and stroke/transient ischemic attack were the common dizziness disorders. The prognosis of most patients with dizziness was good.

Background and objective: Dizziness is a common and challenging symptom, which can be caused by different pathophysiological mechanisms and might affect a large number of population. However, up to now, there have been limited research on the characteristics of dizziness as the chief complaint in hospitalized patients in the Department of Neurology. Thus, the aim of this study was to investigate the hospitalized patients with dizziness as their chief complaint in the Department of Neurology. Methods:In this cross-sectional study, we conducted a retrospective document analysis of hospitalized patients admitted to a tertiary neurological department with the symptom of dizziness during the period of September 2019 to December 2020. We included 211 patients with dizziness as their chief complaint from 1841 patients admitted to this tertiary neurological department during that period.Results: Of all 1841 hospitalized patients, those with dizziness as the chief complaint accounted for 11.5% and most of their past medical history included hypertension, diabetes, cerebrovascular diseases, dyslipidemia, and coronary heart disease. Among these 211 patients, dizziness was more common in women than in men (p = .004). More patients presented with vertigo (40.8%) and light-headedness (39.8%) than disequilibrium (17.1%) and pre-syncope (2.4%). Nausea (48.3%), vomiting (34.1%), headache (13.3%), walking unsteadily (13.3%), and ear symptoms (12.8%) were the most common concomitant symptoms. Dix-Hallpike test (24.6%) and Romberg's sign (11.4%) were positive in these dizzy patients. Nystagmus (2.4%), vision changes (1.4%), and hearing disorders (8.5%) were relatively rare symptoms. Common auxiliary examinations were performed, such as magnetic resonance imaging (60.2%), computed tomography (31.8%), carotid duplex ultrasound (30.8%), and echocardiography (28.0%). Benign paroxysmal positional vertigo (24.2%) and stroke/transient ischemic attack (19.0%) were confirmed to be common causes of dizziness. Note that 97.2% of dizzy patients were in improved recovery after treatment.