A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137(T), was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137(T) was observed to grow at 4-10 °C, at pH 6.5-7.5 and in the presence of 2.5-4.0 % (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137(T) belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824(T) (97.6 %) and Lacinutrix algicola JCM 13825(T) (97.1 %). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137(T) is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5 %) were identified as iso-C15:1 G (19.9 %), iso-C15:0 (19.3 %), iso-C17:0 3-OH (11.3 %), summed feature 9 (C16:0 10-methyl and/or iso-C17:1 ω9c as defined by MIDI, 9.1 %), iso-C15:0 3-OH (7.5 %), and anteiso-C15:1 A (5.8 %). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 32.1 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137(T) is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137(T) (=KCTC 32573(T)=JCM 19883(T)).
A Gram-staining-negative, yellow-pigmented, aerobic, rod-shaped and non-motile bacterium, PAMC 27130 T , was isolated from the marine sediment of the Ross Sea, Antarctica. The temperature, pH and NaCl tolerance ranges for growth were 4-20 6C, pH 6.0-9.0 and 0.5-5.0 % (w/v) NaCl, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PAMC 27130 T belonged to the genus Psychroserpens and was closely related to Psychroserpens mesophilus, Psychroserpens damuponensis and Psychroserpens burtonensis with 97.2, 94.7 and 94.2 % sequence similarities, respectively. Genomic relatedness analyses based on average nucleotide identity and genome-to-genome distance showed that strain PAMC 27130 T could be clearly distinguished from other species of the genus Psychroserpens . The genomic DNA G+C content was 32.7 mol%. The major fatty acids (.10 %) were C 20 : 4 v6c (13.2 %), iso-C 15 : 0 (12.3 %), iso-C 15 : 1 G (11.7 %) and iso-C 15 : 0 3-OH (10.0 %). The major respiratory isoprenoid quinone was menaquinone-6 (MK-6) and the polar lipids were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid, an unidentified aminophospholipid and three unidentified lipids. On the basis of genotypic and phenotypic data collected in this study, it is proposed that strain PAMC 27130 T represents a novel species of the genus Psychroserpens, for which the name Psychroserpens jangbogonensis sp. nov. is proposed. The type strain is PAMC 27130 T (5KCTC 42128 T 5JCM 30228 T ).
Sediminicola arcticus sp. nov., a psychrophilic bacterium isolated from deep-sea sediment, and emended description of the genus Sediminicola A Gram-stain-negative, rod-shaped and non-motile strain, designated PAMC 27266 T , was isolated from deep-sea sediment of the Arctic Ocean. Analysis of the 16S rRNA gene sequence of strain PAMC 27266 T showed closest affiliation with the genus Sediminicola. Phylogenetic analyses revealed that strain PAMC 27266 T formed a robust clade with Sediminicola luteus CNI-3 T , with which it shared 98.9 % 16S rRNA gene sequence similarity. Genomic relatedness analyses based on the average nucleotide identity and genome-to-genome distance showed that strain PAMC 27266 T is clearly distinguished from S. luteus. Cells of strain PAMC 27266 T grew optimally at 15 6C and pH 6.5-7.5 in the presence of 3.5 % (w/v) sea salts. The major polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and two unidentified lipids. The only respiratory quinone was menaquinone-6. The major cellular fatty acids (.10 %) were C 16 : 1 v6c and/or C 16 : 1 v7c and C 15 : 0 . The genomic DNA G+C content was 37.9 mol%. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data presented, we propose strain PAMC 27266 T (5KCCM 43038 T 5JCM 19894 T ) as the type strain of a novel species, with the name Sediminicola arcticus sp. nov.The genus Sediminicola was established by Khan et al. (2006), with Sediminicola luteus as the type species, for a non-motile bacterium isolated from sediment of the shore of the Sea of Japan. At the time of publication of Khan et al. (2006), the genus Sediminicola was found to be phylogenetically distantly related to other valid genera in the family Flavobacteriaceae with low 16S rRNA gene sequence similarities (approximately 88-91 %). The absence of pentadecanoic acid (C 15 : 0 ) from S. luteus CNI-3 T (5NBRC 100966 T ) along with three sister strains corroborated the proposal of a novel genus within the family Flavobacteriaceae (Khan et al., 2006). In this study, a new strain phylogenetically affiliated with the genus Sediminicola was isolated from deep-sea sediment and subjected to a polyphasic taxonomic analysis.A sediment core, ARA03B-15(B)MUC-02, was taken using a multi-core sampler from the Mendeleev Ridge in the western Arctic Ocean (78 u 69 350 N, 175 u 149 20 W; water column depth of 1149 m) during the Araon expedition in August 2012. A surface sample of the sediment core was diluted approximately 50-fold with autoclaved ambient overlying seawater. An aliquot (100 ml) of the sediment slurry was spread onto a plate containing marine agar 2216 (MA; Difco) and the plate was incubated aerobically at 20 u C for 1 week. Strain PAMC 27266 T was isolated on the plate and subsequently purified four times on fresh MA at 20 u C. The strain was maintained both on MA at 4 u C and in marine broth 2216 (MB; Difco) supplemented with 30 % (v/v) glycerol at 280 u C.S. luteus NBRC 100966 T was obtained from NITE Biological Resource Center (NBRC) to compare the p...
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