A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137(T), was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137(T) was observed to grow at 4-10 °C, at pH 6.5-7.5 and in the presence of 2.5-4.0 % (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137(T) belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824(T) (97.6 %) and Lacinutrix algicola JCM 13825(T) (97.1 %). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137(T) is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5 %) were identified as iso-C15:1 G (19.9 %), iso-C15:0 (19.3 %), iso-C17:0 3-OH (11.3 %), summed feature 9 (C16:0 10-methyl and/or iso-C17:1 ω9c as defined by MIDI, 9.1 %), iso-C15:0 3-OH (7.5 %), and anteiso-C15:1 A (5.8 %). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 32.1 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137(T) is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137(T) (=KCTC 32573(T)=JCM 19883(T)).
A Gram-stain-positive, non-motile, strictly aerobic, non-spore-forming and short rod-shaped bacterial strain, CL-GY44 T , was isolated from coastal seawater, Korea. Analysis of the 16S rRNA gene sequence of strain CL-GY44 T revealed a clear affiliation with the genus Nocardioides. Based on phylogenetic analysis, strain CL-GY44 T showed the closest phylogenetic relationship with Nocardioides ginsengagri BX5-10 T and Nocardioides plantarum NCIMB 12834 T . Strain CL-GY44 T was not able to grow in the presence of NaCl but grew with 0-5.5 % sea salts. The optimum temperature and pH for growth were 30 6C and pH 7.0. The major cellular fatty acids of strain CL-GY44 T were C 17 : 1 v6c, iso-C 16 : 0 , C 16 : 0 and iso-C 15 : 0 and the major menaquinone was MK-8(H 4 ). The cell-wall analysis showed that strain CL-GY44 T contained LL-diaminopimelic acid. The genomic DNA G+C content was 71.6 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-GY44 T could be clearly distinguished from members of the genus Nocardioides. Thus, strain CL-GY44 T should be classified as representing a novel species in the genus Nocardioides, for which the name Nocardioides marinquilinus sp. nov. is proposed. The type strain is CL-GY44 T (5KCCM 90109 T 5JCM 18459 T ).The genus Nocardioides, first described by Prauser (1976), belongs to the family Nocardioidaceae, in the suborder Propionibacterineae (Zhi et al., 2009). At the time of writing, the genus Nocardioides comprised 62 species including 58 with validly published names in the List of Prokaryotic Names with Standing in Nomenclature (http://www.bacterio.cict.fr) and four species in press, with names which have been validly published during the processing of this manuscript, Nocardioides albertanoniae isolated from Roman catacombs (Alias-Villegas et al., 2013), Nocardioides perillae isolated from the surface-sterilized root of Perilla frutescens (Du et al., 2013) and Nocardioides szechwanensis and Nocardioides psychrotolerans isolated from Hailuogou glacier in Szechwan, China . Some members in the genus Nocardioides have been isolated from various environments and ten species have been discovered from various marine environments: Nocardioides aestuarii (Yi & Chun, 2004a) Here, we describe a bacterial strain, designated CL-GY44 T , isolated from coastal seawater of Gwangyang Bay, South sea of Korea. Based on the polyphasic approach, we propose that the bacterium represents a novel species in the genus Nocardioides.A sample (10 ml) of coastal seawater was spread on a 1/10-diluted marine agar (MA; Difco) and incubated at 30 u C for 3 weeks. Among 24 strains isolated from 1/10-diluted MA (12 strains of Alpha-proteobacteria, one strain of Betaproteobacteria, five strains of gammaproteobacteria, one strain of Actinobacteria, two strains of Bacteroidetes and three strains of Firmicutes), one strain assigned to the genus Nocardioides showed .3 % 16S rRNA gene sequence divergence from species of the genus Nocardioides with validly published names. A single colon...
Pontimonas salivibrio gen. nov., sp. nov., a new member of the family Microbacteriaceae isolated from a seawater reservoir of a solar saltern A Gram-staining-positive, non-motile, strictly aerobic, non-spore-forming, vibrio-shaped bacterial strain, CL-TW6 T , was isolated from a reservoir seawater sample from a solar saltern in Korea. Analysis of the 16S rRNA gene sequence of strain CL-TW6 T revealed a clear affiliation with the family Microbacteriaceae. Strain CL-TW6 T showed the closest phylogenetic relationships with the genera Yonghaparkia and Microcella, with 16S rRNA gene sequence similarity of 94.8-95.3 %. The strain grew in the presence of 1-9 % sea salts, at 15-35 6C and at pH 7.0-9.0. The major cellular fatty acids of strain CL-TW6 T were anteiso-C 15 : 0 (32.6 %), iso-C 16 : 0 (20.4 %), iso-C 15 : 0 (13.2 %) and iso-C 14 : 0 (11.8 %) and the major menaquinones were MK-9 and MK-10. Cell-wall analysis showed that the peptidoglycan of strain CL-TW6 T contained 2,4-diaminobutyric acid, alanine, glycine and glutamic acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C content of strain CL-TW6 T was 60.0 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed clearly that strain CL-TW6 T could be distinguished from members of the family Microbacteriaceae with validly published names. Thus, strain CL-TW6 T should be classified as representing a novel genus and species in the family Microbacteriaceae, for which the name Pontimonas salivibrio gen. nov., sp. nov. is proposed. The type strain of Pontimonas salivibrio is CL-TW6 T (5KCCM 90105 T 5JCM 18206 T ).
Gracilimonas rosea sp. nov., isolated from tropical seawater, and emended description of the genus Gracilimonas T and G. tropica CL-CB462 T was 6.7 % (reciprocal 9.5 %). Strain CL-KR2 T grew in the presence of 1-20 % sea salts and the optimal salt concentration was 3.5-5 %. The temperature and pH optima for growth were 35 6C and pH 7.5. The major cellular fatty acids (¢10.0 %) of strain CL-KR2 T were iso-C 15 : 0 , summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 v7c) and iso-C 17 : 1 v9c and the only isoprenoid quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, two unidentified glycolipids and two unidentified lipids. The genomic DNA G+C content of strain CL-KR2 T was 43.2 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-KR2 T could be distinguished from the only member of the genus Gracilimonas with a validly published name. Thus, strain CL-KR2 T should be assigned to a novel species in the genus Gracilimonas, for which the name Gracilimonas rosea sp. nov. is proposed. The type strain is CL-KR2 T (5KCCM 90206 T 5JCM 18898 T ).
A Gram-staining-negative, motile, aerobic and rod-shaped bacterial strain, PAMC 27157 T , was isolated from a melt pond on sea ice in the Chukchi Sea. Phylogenetic analysis of the 16S rRNA gene sequence of strain PAMC 27157 T revealed an affiliation to the genus Aureimonas with the closest sequence similarity (96.2 %) to that of Aureimonas phyllosphaerae. Strain PAMC 27157 T grew optimally at 30 8C and pH 7.0 in the presence of 3.5 % (w/v) NaCl. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, sulfoquinovosyldiacylglycerol and an unidentified aminolipid. The major cellular fatty acid was summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c, 83.1 %) and the major respiratory quinone was Q-10. The genomic DNA G+C content was 69
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