Lacinutrix jangbogonensis sp. nov., a psychrophilic bacterium isolated from Antarctic marine sediment and emended description of the genus Lacinutrix

Abstract

A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137(T), was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137(T) was observed to grow at 4-10 °C, at pH 6.5-7.5 and in the presence of 2.5-4.0 % (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137(T) belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824(T) (97.6 %) and Lacinutrix algicola JCM 13825(T) (97.1 %). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137(T) is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5 %) were identified as iso-C15:1 G (19.9 %), iso-C15:0 (19.3 %), iso-C17:0 3-OH (11.3 %), summed feature 9 (C16:0 10-methyl and/or iso-C17:1 ω9c as defined by MIDI, 9.1 %), iso-C15:0 3-OH (7.5 %), and anteiso-C15:1 A (5.8 %). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 32.1 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137(T) is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137(T) (=KCTC 32573(T)=JCM 19883(T)).

Description of Lacinutrix venerupis sp. nov.: A novel bacterium associated with reared clams

“…To date, the genus Lacinutrix comprises three additional species, Lacinutrix algicola and Lacinutrix mariniflava isolated from red algae [13], and Lacinutrix himadriensis isolated from marine sediments [17]. In addition, during the publication process of the present work a new species, Lacinutrix jangbogonensis isolated from Antarctic marine sediment, has been described but it is not yet validated [11]. In the present study, a polyphasic approach was employed in order to characterize and determine the taxonomic affiliation of five unidentified strains, phylogenetically related to the genus Lacinutrix, which could not be assigned to any of the described species in the genus.…”

Beneficial microbiomes: Biodiversity and potential biotechnological applications for sustainable agriculture and human health

“…The low-temperature habitat represents hot spots of biodiversity, and several novel cold-adapted microbial species have been sort out from cold environments including Cellulophaga algicola [40], Cryobacterium roopkundense [41], Exiguobacterium soli [42], Flavobacterium frigidarium [43], Flavobacterium psychrolimnae [44], Glaciimonas frigoris [45], Hymenobacter roseosalivarius [46], Lacinutrix jangbogonensis [47], Massilia eurypsychrophila [48], Octadecabacter arcticus [49], Oleispira antarctica [50], Pedobacter arcticus [51], Pseudomonas extremaustralis [52], Psychrobacter pocilloporae [53], Psychromonas ingrahamii [54], Sphingobacterium psychroaquaticum [55], and Sphingomonas glacialis [56]. Along with novel species of psychrotrophic microbes, some microbial species such as Arthrobacter nicotianae, Brevundimonas terrae, Paenibacillus tylopili, and Pseudomonas cedrina have been isolated and characterized for multifarious PGP attributes at low temperatures from cold deserts of NW Himalayas [7].…”

Draft genome sequence of the psychrophilic bacterium Lacinutrix jangbogonensis PAMC 27137T

“…The genus Lacinutrix which belongs to the phylum Bacteroidetes currently consists of five type species and members of the genus Lacinutrix have been known to produce extracellular protease in marine sediments of Antarctica (Bowman and Nichols, 2005;Nedashkovskaya et al, 2008;Srinivas et al, 2013;Zhou et al, 2013;Lee et al, 2014). Type strains of the genus Lacinutrix were all isolated from copepod, algae, or sediments of polar areas (Bowman and Nichols, 2005;Nedashkovskaya et al, 2008;Srinivas et al, 2013;Lee et al, 2014).…”

“…Type strains of the genus Lacinutrix were all isolated from copepod, algae, or sediments of polar areas (Bowman and Nichols, 2005;Nedashkovskaya et al, 2008;Srinivas et al, 2013;Lee et al, 2014). All type strains of the genus Lacinutrix have been reported to grow at low temperature and exhibit wider growth temperature ranges (from −2-4°C to 20-25°C).…”