Graphical AbstractGraphical abstract of the network meta-analysis. Note: CES-D, Center for Epidemiologic Studies Depression Scale; BDI, Beck Depression Inventory Scale; PHQ-9, the nine-Item Patient Health Questionnaire; K6, Kessler Screening Scale for Psychological Distress; SUCRA, surface area under the cumulative ranking curves.
Background: The pathogenesis of ulcerative colitis (UC) is closely related to immunity. The immune characteristic differences between active UC (UCa) and inactive UC (UCin) have not been completely explained. Mass cytometry (CyTOF) and single-cell RNA sequencing (scRNA-seq) were used to analyze the immune cells of UCa, UCin and healthy control (HC) subjects to determine the specific immune characteristics.Methods: The immune cell subsets among UCa, UCin, HC were distinguished using CyTOF analysis. scRNA-seq analysis was used to validate the results of CyTOF. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to understand the roles of differential immune cell subsets.Results: After CyTOF analysis and validation of scRNA-seq analysis, differential immune cell subsets mainly contained TNF+IL-17A++ effector memory (EM) Tregs, CXCR3+CTLA4+ EM Tregs, CXCR3++CCR7+ B cells, HLA-DR+CCR7+ dendritic cells (DCs) and CTLA-4+ natural killer (NK) cells. In comparison to HC, CCR6+TNF+CD161+ EM T cells were highly enriched in UCa and UCin. Besides, UCa was characterized by an increase in CD38+TNF+ EM Tregs, CXCR3+CCR4+ naïve B cells, HLA-DR+CD14+IL21+ macrophages/monocytes, HLA-DR+CCR7+ DCs, AHR+CD14+ cytotoxic NK (cNK) cells and CD8A+IFNG+ cNK cells. Decreases in CD38+CD27+ plasmablasts, CXCR3+CD38+ regulatory NK cells, and CXCR3+CCR7+ tolerant NK cells in UCa were discovered.Conclusions: Novel immune cell subsets which was used to distinguish UCa, UCin and HC were identified. This information might be utilized to distinguish the patients with UCa and UCin.
ObjectivesFor Crohn’s disease (CD), the alternation of the active phase and inactive phase may be related to humoral immunity and cellular immunity. This study aims to understand the characteristics of immune cells in patients with active CD (CDa) and inactive CD (CDin).MethodsMass cytometry (CyTOF) and single-cell RNA sequencing (scRNA-seq) data about CDa, CDin, and healthy control (HC) were included. CyTOF analysis was performed to capture gated subsets, including T cells, T regulatory (Treg) cells, B cells, innate immune cells, and natural killer (NK) cells. Differential analysis was used to identify different immune cell subsets among CDa, CDin, and HC. ScRNA-seq analysis was used to verify the results of CyTOF. CD-related signaling pathways were obtained using KEGG pathway enrichment analysis. CellChat analysis was used to infer the cell communication network among immune cell subsets.ResultsCompared to patients with CDin, patients with CDa had higher abundances of CD16+CD38+CD4+CXCR3+CCR6+ naive T cells, HLA-DR+CD38+IFNγ+TNF+ effector memory (EM) T cells, HLA-DR+IFNγ+ naive B cells, and CD14++CD11C+IFNγ+IL1B+ monocytes. KEGG analysis showed the similarity of pathway enrichment for the earlier four subsets, such as thermogenesis, oxidative phosphorylation, and metabolic pathways. The patients with CDin were characterized by an increased number of CD16+CD56dimCD44+HLA-DR+IL22+ NK cells. Compared to HC, patients with CDa demonstrated a low abundance of HLA-DR+CCR6+ NK cells and a high abundance of FOXP3+CD44+ EM Tregs. CellChat analysis revealed the interaction network of cell subsets amplifying in CDa compared with CDin.ConclusionSome immune subsets cells were identified for CDa and CDin. These cells may be related to the occurrence and development of CD and may provide assistance in disease diagnosis and treatment.
Background: The aim of our study was to translate and validate the Chinese version of the Short Health Scale (SHS), a disease-specific quality of life (QoL) scale for the patients with inflammatory bowel disease (IBD).Methods: The SHS was translated and validated according to the standard process: a translation and back-translation procedure, culture adaptation and a validation study. Patients with IBD were enrolled, and their QoL were assessed using the SHS and the short inflammatory bowel disease questionnaire (SIBDQ). Reliability (internal consistency reliability, split-half reliability and test-retest reliability) and validity (content validity, construct validity, criterion validity and discriminant validity) analysis were performed to evaluate the psychometric characteristics of the SHS.Results: A total of 95 patients with IBD (62 ulcerative colitis and 33 Crohn’s disease) completed the Chinese version of the SHS, and 40 patients completed the SHS within 1-4 weeks once again. Cronbach's alpha value of the SHS was 0.91, and its split-half coefficient was 0.83. Intraclass correlation coefficients of four items ranged from 0.55 to 0.75. All four items of the SHS were significantly associated with the corresponding domains of the SIBDQ, with correlation coefficients ranging from -0.47 to -0.63 (P < 0.001). Exploratory factor analysis showed that the cumulative contribution rate of variance reached 68%, and the factor loading of all the items were greater than 0.8. The scores of four items were significantly different for the patients of different Bristol stool form scale (P < 0.001). The scores of function, worry and general well-being were significantly different among the patients with different smoking status (P < 0.05).Conclusions: The SHS is a simple and quick scale. The SHS had good validity and reliability, and was suitable to evaluate the QoL of patients with IBD in Chinese.
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