Selenium nanoparticles (Se NPs) possess well-known excellent biological activities and low toxicity, and have been employed for numerous applications except as inhibitors to protein glycation. Herein, the present study is carried out to investigate the inhibitory effect of Se NPs on protein glycation in a bovine serum albumin (BSA)/glucose system. By measuring the amount of glucose covalently bound onto BSA, the formation of fructosamine and fluorescent products, it is found that Se NPs can hinder the development of protein glycation in a dose-dependent but time-independent manner under the selected reaction conditions (55 °C, 40 h). And after comparing the increase of inhibitory rate in different stages, it is observed that Se NPs show the greatest inhibitory effect in the early stage, then in the advanced stage, but no effect in the intermediate stage. Fourier transform infrared spectroscopy characterization of Se NPs collected after glycation and determination of ·OH influence and glyoxal formation show that the mechanism for the inhibitory efficacy of Se NPs is related to their strong competitive activity against available amino groups in proteins, their great scavenging activity on reactive oxygen species and their inhibitory effect on α-dicarbonyl compounds' formation. In addition, it is proved that Se NPs protect proteins from structural modifications in the system and they do not exhibit significant cytotoxicity towards BV-2 and BRL-3A cells at low concentrations (10 and 50 μg mL(-1)). Consequently, Se NPs may be suitable for further in vivo studies as novel anti-glycation agents.
Although numerous studies have been paid much attention to rainfall-induced instability of multilayered slopes, the interface between layers is generally considered to be “zero thickness”, and the layer transition zone between layers is neglected. In this study, the influence of the layer transition zone on the rainfall-induced instability of multilayered slope was investigated. A model was developed to simulate the rainfall infiltration process, the distribution of pore water pressure, and the stability of multilayered slope by coupling the unsaturated seepage model and the slope stability analysis method. Based on the analysis of the multilayered slopes with the different thickness ratios of the layer transition zone, a method for determining the critical thickness of the layer transition zone was proposed. The results showed that the layer transition zone had a significant influence on the stability of multilayered slope. It was found that the presence of the layer transition zone in the multilayered slope reduced the hydraulic conductivity of the slope and increased the rate of formation of transient saturated zone, which contributed to excess pore water pressure at the toe of the slope. The analysis of the local factor of safety (LFS) showed that when the thickness ratios of the layer transition zone were between 2.5% and 5%, the corresponding hydraulic conductivity of the slope decreased by 1%-2.5% and the maximum failure area of the slope during the rainfall was 25% of the slope. Our study highlighted the importance of the layer transition zone for the rainfall-induced instability of the multilayered slope.
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