The inhibitory effect of selenium nanoparticles on protein glycation<i>in vitro</i>

Yu, Shaoxuan; Zhang, Wentao; Liu, Wei; Zhu, Wenxin; Guo, Ruochen; Wang, Yashan; Zhang, Daohong; Wang, Jianlong

doi:10.1088/0957-4484/26/14/145703

Cited by 36 publications

(32 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alternative explanation for decreased levels of HbA1c upon Se is the possibility that Se may prevent the process of hemoglobin glycation. This may be suggested on the basis of a recent study by Yu and colleagues, showing Se nanoparticles prevent protein glycation in vitro [40]. Nevertheless, it is not clear whether changes observed in our study at the level of leukocyte gene expression reflect changes in insulin-sensitive tissues.…”

Section: Discussionmentioning

confidence: 73%

The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism

et al. 2016

View full text Add to dashboard Cite

The aim of the study was to evaluate the effect of selenium supplementation on the expression of genes associated with glucose metabolism in humans, in order to explain the unclear relationship between selenium and the risk of diabetes. For gene expression analysis we used archival samples of cDNA from 76 non-diabetic subjects supplemented with selenium in the previous study. The supplementation period was six weeks and the daily dose of selenium was 200 µg (as selenium yeast). Blood for mRNA isolation was collected at four time points: before supplementation, after two and four weeks of supplementation, and after four weeks of washout. The analysis included 15 genes encoding selected proteins involved in insulin signaling and glucose metabolism. In addition, HbA1c and fasting plasma glucose were measured at three and four time points, respectively. Selenium supplementation was associated with a significantly decreased level of HbA1c but not fasting plasma glucose (FPG) and significant down-regulation of seven genes: INSR, ADIPOR1, LDHA, PDHA, PDHB, MYC, and HIF1AN. These results suggest that selenium may affect glycemic control at different levels of regulation, linked to insulin signaling, glycolysis, and pyruvate metabolism. Further research is needed to investigate mechanisms of such transcriptional regulation and its potential implication in direct metabolic effects.

show abstract

Section: Discussionmentioning

confidence: 73%

The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism

et al. 2016

View full text Add to dashboard Cite

show abstract

“…5). 19 There was an increase in endogenous SeNPs between 24 h and 36 h, and a decrease between 36 h and 72 h according to UV-Vis measurements (Fig. 5).…”

Section: Dynamic Equilibrium Of Intracellular Senp Assemblymentioning

confidence: 79%

“…The wavelength scanning was performed from 230 to 500 nm at 1.0 nm wavelength resolution, and SeNPs were monitored at around 275 nm. 19 In quartz cuvettes, the standards for SeNPs were prepared in 50 mM Tris-HCl buffer (pH 7.0) supplemented with BSA, 0.5 mM selenite and 4 mM reduced glutathione. 20 …”

Section: Extraction and Uv-vis Spectroscopy Of Senpsmentioning

confidence: 99%

Dynamic equilibrium of endogenous selenium nanoparticles in selenite-exposed cancer cells: a deep insight into the interaction between endogenous SeNPs and proteins

2015

View full text Add to dashboard Cite

Elemental selenium (Se) was recently found to exist as endogenous nanoparticles (i.e., SeNPs) in selenite-exposed cancer cells. By sequestrating critical intracellular proteins, SeNPs appear capable of giving rise to multiple cytotoxicity mechanisms including inhibition of glycolysis, glycolysis-dependent mitochondrial dysfunction, microtubule depolymerization and inhibition of autophagy. In this work, we reveal a dynamic equilibrium of endogenous SeNP assembly and disassembly in selenite-exposed H157 cells. Endogenous SeNPs are observed both in the cytoplasm and in organelles. There is an increase in endogenous SeNPs between 24 h and 36 h, and a decrease between 36 h and 72 h according to transmission electron microscopy results and UV-Vis measurements. These observations imply that elemental Se in SeNPs could be oxidized back into selenite by scavenging superoxide radicals and ultimately re-reduced into selenide; then the assembly and disassembly of SeNPs proceed simultaneously with the sequestration and release of SeNP high-affinity proteins. There is also a possibility that the reduction of elemental Se to selenide pathway may lie in selenite-exposed cancer cells, which results in the assembly and disassembly of endogenous SeNPs. Genome-wide expression analysis results show that endogenous SeNPs significantly altered the expression of 504 genes, compared to the control. The endogenous SeNPs induced mitochondrial impairment and decreasing of the annexin A2 level can lead to inhibition of cancer cell invasion and migration. This dynamic flux of endogenous SeNPs amplifies their cytotoxic potential in cancer cells, thus provide a starting point to design more efficient intracellular self-assembling systems for overcoming multidrug resistance.

show abstract

“…It was found that addition of Se NPs together with glucose to a BSA solution significantly decreased the development of protein glycation. 108 As Se NPs have large surface to volume ratio BSA has already bond upon the surfaces of Se NPs, thus, deceases the reactivity of glucose molecule towards the amino acids of BSA and consequently inhibiting protein glycation. Se NPs inhibit the protein glycation in a dose-dependent but time-independent manner under the selected reaction conditions.…”

Section: Vii) Inhibitor Of Protein Glycationmentioning

confidence: 99%

Selenium nanostructures: microbial synthesis and applications

Shirsat¹,

Kadam²,

Naushad

et al. 2015

RSC Adv.

View full text Add to dashboard Cite

Due to considerable impact on day-to-day life through superior functioning of energy, medicine, electronics, sensors and space industries young researchers are being attracted towards nanotechnology/biotechnology field. Selenium (Se) is acting as an essential element in most of the organism and beneficial nutrient in higher plants. Se nanostructures (NSs) i.e. nanospheres or particles (NPs) and nanowires (NWs) have unusual properties compared to their bulk counterparts. It has seven-fold lower toxicity than its other biologically utilizable materials. Several physical & chemical methods are being applied for the synthesis of Se NSs, but development of eco-friendly routes is of considerable importance towards biological applications. Recently microorganisms are being reported for the synthesis of Se NSs by reduction of oxyions in anaerobic and aerobic environments. This review briefly proposes the recent developments in the microbial synthesis, characterization, synthesis mechanism of Se NSs along with non-biological (solar, sensor, photocatalysis etc.) and biological (anticancer, antioxidant, antiprotozoal etc.) applications. Advantages of Se NSs synthesized using green approaches, over commonly used, are explored.

show abstract

The inhibitory effect of selenium nanoparticles on protein glycationin vitro

Cited by 36 publications

References 88 publications

The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism

The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism

Dynamic equilibrium of endogenous selenium nanoparticles in selenite-exposed cancer cells: a deep insight into the interaction between endogenous SeNPs and proteins

Selenium nanostructures: microbial synthesis and applications

Contact Info

Product

Resources

About