Selenium nanostructures: microbial synthesis and applications

Shirsat, Shubhangi D.; Kadam, Ambadas S.; Naushad, Mu.; Mane, Rajaram S.

doi:10.1039/c5ra17921a

Cited by 68 publications

(51 citation statements)

References 101 publications

(77 reference statements)

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“…It turned out that SeNPs obtained using microorganisms have a negative zeta potential which causes a small tendency towards agglomeration, i.e. SeNPs exhibited greater resistance to maintaining the degree of dispersion (Shirsat et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of effects of selenium nanoparticles on Bacillus subtilis

Tymoshok

Kharchuk

Kaplunenko

et al. 2019

Regul. Mech. Biosyst.

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The present study was performed to characterize of selenium nanoparticles (Nano-Se) which were synthesized by pulsed laser ablation in liquids to obtain the aqueous selenium citrate solution. The study was conducted using bacteriological and electronic-microscopic methods. Transmission electron microscopy (TEM) and spectroscopy analyses demonstrated that nano-selenium particles obtained by the method of selenium ablation had the size of 4–8 nm. UV-Visible Spectrum colloidal solution Nano-Se exhibited absorption maxima at 210 nm. To clarify some effects of the action of Nano-Se on Bacillus subtilis, we investigated the interaction of Nano-Se with B. subtilis IMV B-7392 before and after incubation with Nano-Se, examining TEM images. It has been shown that exposure to B. subtilis IMV B-7392 in the presence of Nano-Se is accompanied by the rapid uptake of Nano-Se by bacterial culture. TEM analysis found that the electron-dense Nano-Se particles were located in the intracellular spaces of B. subtilis IMV B-7392. That does not lead to changes in cultural and morphological characteristics of B. subtilis IMV B-7392. Using TEM, it has been shown that penetration of nanoparticles in the internal compartments is accompanied with transient porosity of the cell membrane of B. subtilis IMV B-7392 without rupturing it. The effective concentration of Nano-Se 0.2 × 10–3 mg/mL was found to increase the yield of biologically active substances of B. subtilis. In order to create probiotic nano-selenium containing products, the nutrient medium of B. subtilis IMV B-7392 was enriched with Nano-Se at 0.2 × 10–3 mg/mL. It was found that particles Nano-Se are non-toxic to the culture and did not exhibit bactericidal or bacteriostatic effects. The experimentally demonstrated ability of B. subtilis to absorb selenium nanoparticles has opened up the possibility of using Nano-Se as suitable drug carriers.

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Section: Introductionmentioning

confidence: 99%

Evaluation of effects of selenium nanoparticles on Bacillus subtilis

Tymoshok

Kharchuk

Kaplunenko

et al. 2019

Regul. Mech. Biosyst.

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“…Selenium in nano-dimensions has gained tremendous attention and are high in demand due to its extensive applications in electronics, agriculture, food, feed and environmental bioremediation along with a special emphasis in the eld of medicine due to its crucial biological role even at low concentration (Shirsat et al 2015). The speci city, selectivity, bioavailability and low toxicity of SeNPs make them one of the most promising candidate to be used for biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Halomonas Venusta Mediated Detoxification and Biotransformation of Selenite Into Selenium Nanoparticles Exhibiting Various Biomedical Applications

Vaigankar¹,

Dubey²,

Mujawar³

et al. 2020

Preprint

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Marine environment is in constant threat due to anthropogenic activities which are involved in disturbing the aquatic flora and fauna due to accumulation of toxic metals and metalloids. The current study involves the use of microbial remediation strategy for reduction of toxic sodium selenite (Na2SeO3) into less toxic elemental Se (Se0) with concurrent synthesis of Se nanoparticles (SeNPs) possessing several biomedical potential. Selenite reducing bacterial strain isolated from Mandovi estuary of Goa, India was identified as Halomonas venusta based on 16S rRNA gene sequence analysis and designated as strain GUSDM4. It's maximum tolerance level for Na2SeO3 was 100 mM. The 2, 3-diaminonaphthalene based spectroscopic analysis clearly demonstrated 93% reduction of 4 mM Na2SeO3 to Se0 during late stationary growth phase of Halomonas venusta. Biosynthesis of SeNPs commenced within 4 h during log phase which was clearly evident from red colour in the growth medium and a characteristic peak at 265 nm revealed by UV-Vis spectrophotometry. The intracellular synthesis of SeNPs was confirmed by transmission electron microscopy (TEM) of these bacterial cells. Characterization of SeNPs by X-ray crystallography, TEM and energy dispersive X-ray analysis clearly demonstrated spherical SeNPs of 20-80 nm diameter with hexagonal crystal lattice. These SeNPs at 50 µg/mL exhibited 90% free radical scavenging activity and also demonstrated anti-biofilm activity at 20 µg/mL against common human pathogens which was evident by SEM analysis. These SeNPs interestingly revealed excellent dose-dependent and selective anti-proliferative activity against A549 cancer cell line and mosquito larvicidal activity against Aedes aegypti, Culex quinquefasciatus and Anopheles stephensi. Therefore, these studies have demonstrated amazing potential of marine bacterium, Halomonas venusta in bioremediation along with biosynthesis of SeNPs and their applications as free radical scavenger, anti-biofilm, chemo-therapeutic and larvicidal agents which is the first report of its kind.

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“…All of the microorganisms studied so far have synthesized only polydisperse nanoparticles with sizes ranging from 50 nm to 500 nm, with the average size being 100 nm (Dhanjal & Cameotra 2010;Kuroda et al 2011;Prakash et al 2009). The major challenges in the biogenic production method are deprived product quality, i.e., higher polydispersity index and larger size along with the need for meticulous postproduction treatment (Shirsat et al 2015). Nevertheless, literature gives us enough indications suggesting that the challenges can be addressed by understanding the mechanism of action involved in the biogenic production of SeNPs.…”

Section: Application Of Senps 49mentioning

confidence: 99%

“…There are a variety of applications of SeNPs in industry, the growth of some crops, prevention and treatment of certain diseases including cancer, as well as antifungal activities (Shirsat et al 2015). The most prominent feature of the SeNPs is reducing oxidative stress through antioxidant actions which are useful in the treatment of cancers and heart diseases (Chen et al 2008;Flohé 2011;El-Batal et al 2012).…”

Section: Application Of Senps 49mentioning

confidence: 99%

“…Indeed, the peaks of from those of the bacterial biomass (controls) and the Chem-SeNPs. The main difference between the spectra is that the Bio-SeNPs exhibit more peaks in the polysaccharide vibration region, indicating the presence of proteins and polysaccharides in the biomacromolecules capping the SeNPs (Shirsat et al 2015;Jain et al 2015b;Wadhwani et al 2016;Kamnev et al 2017;.…”

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confidence: 99%

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Bioremediation of selenium species in solution by methanotrophic bacteria

Eswyah¹

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Selenium species, particularly the oxyanions selenite and selenate, are significant pollutants in the environment that leach from rocks and are also released by anthropogenic activities. In the environment, microbial transformations of selenium play important roles in the biogeochemical cycles of the element. For instance, microbial reduction of the toxic and water-soluble selenium oxyanions to nanoparticulate elemental selenium greatly reduces the toxicity and bioavailability of selenium and has a major role in bioremediation. Also, microbial methylation after reduction of selenium oxyanions is another potentially effective detoxification process if limitations with low reaction rates and capture of the volatile methylated selenium species can be overcome. Methane-oxidizing bacteria are well known for their role in the global methane cycle and their potential for microbial transformation of wide range of hydrocarbon and chlorinated hydrocarbon pollution. Recently, it has also emerged that methaneoxidizing bacteria interact with inorganic pollutants in the environment. Here, the selenium-transforming properties of methane-oxidizing bacteria have been investigated for the first time. The interaction of selenium containing chemical species has been studied with pure strains of the commonly used laboratory model strains of methane-oxidizing bacteria, Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b. The two strains were both able to convert the toxic selenite but not selenate or DLselenocystine to extracellular red spherical nanoparticulate elemental selenium, which was confirmed by X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine structure (EXAFS). The selenium nanoparticles were characterized by a variety of techniques, including transmission electron microscopy (TEM) energy dispersive X-ray (EDX) spectrometry, high-angle annular dark-field (HAADF) scanning TEM (STEM), X-ray photoelectron spectroscopy (XPS), SDS-PAGE analyses, Fouriertransform infrared spectroscopy (FTIR), zeta potential and Raman spectroscopy. The results showed that the reduction process is an enzymatic reaction and mediated by cell wall-associated proteins. The elemental red selenium nanoparticles formed during selenite reduction were found to be amorphous containing a certain amount of sulfur. ii The results also indicated that the produced selenium nanoparticles are coated with organic materials, likely to be proteins and extracellular polymeric substances (EPS). The cultures also produced volatile selenium-containing species when challenged with selenite, which shows that both strains have an additional activity that can transform either elemental selenium or selenite into volatile methylated forms of selenium, including dimethyl selenide, dimethyl diselenide, dimethyl selenenyl sulphide, methylselenol and methylselenoacetate. Selenate (at concentrations higher than 100 µg mL-1) or DL-selenocystine-amended cultures of Methylococcus capsulatus (Bath) (but not Methylosinus trichosporium OB3b) p...

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Selenium nanostructures: microbial synthesis and applications

Cited by 68 publications

References 101 publications

Evaluation of effects of selenium nanoparticles on Bacillus subtilis

Evaluation of effects of selenium nanoparticles on Bacillus subtilis

Halomonas Venusta Mediated Detoxification and Biotransformation of Selenite Into Selenium Nanoparticles Exhibiting Various Biomedical Applications

Bioremediation of selenium species in solution by methanotrophic bacteria

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