Evaluation of effects of selenium nanoparticles on Bacillus subtilis

Abstract

The present study was performed to characterize of selenium nanoparticles (Nano-Se) which were synthesized by pulsed laser ablation in liquids to obtain the aqueous selenium citrate solution. The study was conducted using bacteriological and electronic-microscopic methods. Transmission electron microscopy (TEM) and spectroscopy analyses demonstrated that nano-selenium particles obtained by the method of selenium ablation had the size of 4–8 nm. UV-Visible Spectrum colloidal solution Nano-Se exhibited absorption maxima at 210 nm. To clarify some effects of the action of Nano-Se on Bacillus subtilis, we investigated the interaction of Nano-Se with B. subtilis IMV B-7392 before and after incubation with Nano-Se, examining TEM images. It has been shown that exposure to B. subtilis IMV B-7392 in the presence of Nano-Se is accompanied by the rapid uptake of Nano-Se by bacterial culture. TEM analysis found that the electron-dense Nano-Se particles were located in the intracellular spaces of B. subtilis IMV B-7392. That does not lead to changes in cultural and morphological characteristics of B. subtilis IMV B-7392. Using TEM, it has been shown that penetration of nanoparticles in the internal compartments is accompanied with transient porosity of the cell membrane of B. subtilis IMV B-7392 without rupturing it. The effective concentration of Nano-Se 0.2 × 10–3 mg/mL was found to increase the yield of biologically active substances of B. subtilis. In order to create probiotic nano-selenium containing products, the nutrient medium of B. subtilis IMV B-7392 was enriched with Nano-Se at 0.2 × 10–3 mg/mL. It was found that particles Nano-Se are non-toxic to the culture and did not exhibit bactericidal or bacteriostatic effects. The experimentally demonstrated ability of B. subtilis to absorb selenium nanoparticles has opened up the possibility of using Nano-Se as suitable drug carriers.

Biosynthesis of Selenium Nanoparticles (via Bacillus subtilis BSN313), and Their Isolation, Characterization, and Bioactivities

“…At the concentration of 100 µg/mL (Se in the medium), more than95% ionic Se was reduced into Se 0 after 48 h. During the log phase of Bacillus subtilis BSN313, the reduction phase (Se 0 ) occurred. Same kind of observations were recorded in the same genus, reported in the previous literature [68,70,95]. However, the total reduction occurred, when the concentration of the selenium in the medium was increased to 200 µg/mL.…”

Efficiency of application of inorganic and nanopreparations of selenium and probiotics for growing young quails

“…Such results are associated with easier absorption of bio nano-Se, in contrast to sodium selenite, in the quails' gastrointestinal tract and more intensive inclusion of the former in metabolism, in particular in the enzymes of antioxidant protection (glutathionedependent enzymes) and thyroid hormones. receiving bio nano-Se, show higher gains and body weight (Bityutskyy et al, 2019b;Tymoshok et al, 2019;Tsekhmistrenko et al, 2020a;2020b).…”

“…Due to the high demand for poultry products, it is necessary to apply the latest technologies for poultry farming, increase its productivity without reducing the quality of the final product, ensure intensive metabolism in farmed livestock, and increased stress resistance and resistance to adverse environmental factors (Bityutskyy et al, 2019a;Tsekhmistrenko et al, 2020a). That is why it is appropriate to use biologically active substances (Tsekhmistrenko et al, 2018) and probiotics (Tymoshok et al, 2019) in a balanced diet, which allow obtaining high-quality products from healthy livestock without the use of feed antibiotics (Tsekhmistrenko et al, 2020b).…”

Bacterial synthesis of nanoparticles: A green approach

“…CKCr-6A (Mesbahi-Nowrouzi & Mollania, 2018), Alishewanella sp. WH16-1 (Xia et al, 2018), Васillus subtilis IMV B-7392 (Tymoshok et al, 2019) were used.…”