Exploring
new functions of nanomaterials can help facilitate the
development of biosensors for the detection of antibiotics. Herein,
a new detection modality based on monovalent antigen-induced aggregation
(MAA) of immunomagnetic beads (IMBs) was proposed for rapid and label-free
detection of enrofloxacin (ENR), which endowed IMBs with the abilities
of both sample separation and signal generation. In the presence of
ENR, the initially well-dispersed IMBs were aggregated and the degree
of aggregation was in a concentration-dependent manner. After exploring
the mechanism underlying IMB aggregation and investigating the key
parameters affecting it, a label-free biosensing platform was developed
for rapid and sensitive detection of ENR. Based on the significant
differences in the magnetic separation speed and size between the
aggregated and well-dispersed IMBs, two methods were proposed for
quantitatively determining ENR, i.e., measuring the turbidity of the
IMB supernatant after magnetic separation for a given time and visualizing
and calculating the grayscale value of the aggregated IMBs trapped
on the surface of a nitrocellulose membrane. A three-dimensional (3D)-printed
syringe was designed and fabricated for automatic filtration of IMBs.
This immunosensor allowed for sensitive detection of ENR in less than
15 min without any labels. It exhibited a satisfactory limit of detection
of 0.79 ng mL–1 and showed the feasibility for ENR
detection of spiked chicken meat with recovery rates ranging from
74.8 to 98.3%. The MAA immunosensor can act as a promising tool to
detect trace levels of ENR and has the potential to be applied to
complex food samples.
Antibiotic residues in foods pose a serious threat to human health. However, routine analysis techniques require bulky laboratory instruments and skilled personnel or give single-channel analysis results, exhibiting low practicality....
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