Fluorescence Nanobiosensor for Simultaneous Detection of Multiple Veterinary Drugs in Chicken Samples

Peng, Yaping; He, Yawen; Shen, Yafang; Liang, Aoming; Zhang, Xibin; Liu, Yuanjie; Lin, Jianhan; Wang, Jianping; Li, Yanbin; Fu, Yingchun

doi:10.1007/s41664-021-00199-4

Cited by 15 publications

(2 citation statements)

References 46 publications

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“…qPCR was performed using a LightCycler 96 (Roche, Basel, Switzerland) targeting 8 ARGs commonly found in domestic wastewater including genes conferring resistance to sulfonamides ( sul 1 and sul 2), macrolides ( erm B and erm F), β-lactams ( amp C and oxa -1), and tetracycline ( tet O and tet W), as well as a class 1 integron gene ( intI 1), and a single gene copy per cell biomarker ( rpo B). − All qPCR reactions were carried out in 20 μL reactions containing 10 μL qPCR master mix (Forget-Me-Not EvaGreen, Biotium, Fermont, CA), 1 μL each of forward and reverse primers (0.25 μM final concentration), 1 μL of DNA template, and 7 μL of nuclease-free water. Each reaction was performed in triplicates.…”

Section: Materials and Methodsmentioning

confidence: 99%

Metagenomic Analysis of the Antibiotic Resistance Risk between an Aerobic and Anaerobic Membrane Bioreactor

et al. 2022

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Membrane bioreactors are advanced treatment technologies that, compared to conventional treatment processes, are expected to reduce antibiotic resistance spread in the environment due to their superior biomass retention. In this study, metagenomic sequencing was used to assess the response of the microbial community and antibiotic resistance profile of an aerobic membrane bioreactor (AeMBR) and an anaerobic membrane bioreactor (AnMBR) operated in parallel, treating identical synthetic wastewater supplemented with select antibiotics. Our results revealed a greater antibiotic impact on the microbial community and antibiotic resistance gene (ARG) proliferation within the AeMBR compared to the AnMBR. Moreover, antibiotic loading elicited a more pronounced disruption of the microbial diversity of the AeMBR biomass and effluent intracellular DNA compared to its AnMBR counterpart. Under antibiotic loading, the AeMBR effluent enriched ARGs in the form of antibiotic-resistant bacteria, while the AnMBR effluent enriched a wide set of extracellular ARGs across a broad spectrum of resistance classes. These results provide a theoretical basis for process selection and controlling the spread of ARGs during wastewater management.

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Section: Materials and Methodsmentioning

confidence: 99%

Metagenomic Analysis of the Antibiotic Resistance Risk between an Aerobic and Anaerobic Membrane Bioreactor

et al. 2022

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“…As is known, fluorescence-based techniques attract wide research interest because of the low background and strong interference rejection. Practically, organic molecules with high reactivity toward a certain target have been widely applied in the design of analytical probes in the last decades by using organic substrates. − It has been found that the substitution of organic molecules and nanomaterials with various functional groups and/or single atoms largely affects their chemical performances including catalysis, optical property, energy storage, and so on. − For instance, Gessner group have found that ylide-substituted phosphines can be used as strong donor ligands for metal-catalyzed coupling reactions . Similarly, Kosgei et al have reported that the substitution of ruthenium phosphine sulfoxide complexes is critical to their photoisomerization reactivity .…”

mentioning

confidence: 99%

3,5-Dihydroxybenzoic Acid-Based Selective Dopamine Detection via Subsititution-Enhanced Kinetics Differences

Liu,

Cheng,

et al. 2023

Anal. Chem.

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The selective recognition of dopamine (DA) over other neurotransmitter analogues is difficult due to the similar molecular structure and chemical reactivity. In this study, substitution-regulated chemical reactivity of the sensing substrate is utilized to explore a novel DA detection probe with satisfying selectivity. As a case study, 3,5-dihydroxybenzoic acid (DHBA, carboxy-substituted resorcinol)-based probes have been explored for selective and ratiometric DA sensing. The carboxy substitution benefits the stabilization of the carbanion intermediate and the azamonardine product, which enhances the reaction kinetics and thermodynamics and subsequently facilitates selective DA recognition over other analogues and interferents. By exploring DHBA emission as the internal reference, ratiometric fluorescence variation is realized, which contributes to sensitive DA analysis. With the combination of logic gate and fluorometric analysis, DA detection in both low and high concentrations can be readily achieved. In addition, the DA analysis in biological samples and the enzymatic transformation of DA analogues in cerebrospinal fluid samples are achieved by the proposed DHBA probe.

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The Impact of Ultrasonication on SDS-stabilized Silver Nanoparticles as a Probe for Trace Determination of Diaveridine

Kassem

El-Feky

2022

Plasmonics

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Fluorescence Nanobiosensor for Simultaneous Detection of Multiple Veterinary Drugs in Chicken Samples

Cited by 15 publications

References 46 publications

Metagenomic Analysis of the Antibiotic Resistance Risk between an Aerobic and Anaerobic Membrane Bioreactor

Metagenomic Analysis of the Antibiotic Resistance Risk between an Aerobic and Anaerobic Membrane Bioreactor

3,5-Dihydroxybenzoic Acid-Based Selective Dopamine Detection via Subsititution-Enhanced Kinetics Differences

The Impact of Ultrasonication on SDS-stabilized Silver Nanoparticles as a Probe for Trace Determination of Diaveridine

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