Summary.Radix Isatidis has widely useful activities including anti-virus, anti-bacterial. Tryptanthrin, indigo, and indirubin are active ingredients in R. Isatidis. Response surface methodology (RSM)-optimized infrared-assisted extraction (IRAE) was developed and combined with HPLC for simultaneous determination of tryptanthrin, indigo, and indirubin from R. Isatidis. IRAE were investigated through extraction yields of the three components and optimized by RSM. The optimum conditions were as follows: infrared power of 129 W, solid/liquid ratio of 1:40 g/mL, and irradiation time of 22.5 min. IRAE conditions obtained by RSM were not only accurate, but also had practical value reflecting the expected optimization. Subsequently, this novel IRAE method was evaluated by extraction yield of the components of R. Isatidis samples from different regions. Compared with common extraction methods including maceration extraction (ME), reflux extraction (RE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE), IRAE showed higher yield with advantages of no limitation of solvent selection, low cost, convenience under optimum extraction conditions. These results suggested the potential of RSM-optimized IRAE for extraction and analysis of the water-/fat-soluble compositions of Chinese herbal medicine. A simple chromatographic separation for simultaneous determination of tryptanthrin, indigo, and indirubin from Chinese herbal medicine R. Isatidis was performed on a C 18 column (Diamonsil 150 mm × 4.6 mm i.d., 5 μm) with a mobile phase isocratic consisting of methanol and water at a flow-rate of 0.8 mL min −1 . The retention times of tryptanthrin, indigo, and indirubin were 15.4, 31.9, and 58.6 min, respectively. The linear equations were obtained as follows: y = −3094.5744 + 21208.792x for tryptanthrin (R = 0.9998, 0.9-18.0 μg mL −1 ), y = 4730.0448 + 30180.567x for indigo (R = 0.9997, 0.5-10.0 μg mL −1 ) and y = −6582.9045 + 67069.312x for indirubin (R = 0.9997, 0.4-8.0 μg mL −1 ). The result showed that RSM-optimized IRAE was a simple, efficient pretreatment method for the analysis of complex matrix.
In this study, we successfully studied water-soluble extract from Radix isatidis. Optimized conditions of MAE were listed, the sample can be extracted completely in 10 minutes under microwave power of 400W and solid/liquid ratio of 1:80. Active compounds in water-soluble extract from R. isatidis were identified with HPLC-DAD/ESI-MS, these compounds followed by cytidine, uridine, guanosine, (R,S)-goitrin and adenosine. RODWs-HPLC as a new sensitive chromatography were also first proposed and investigated, we favoringly used this method for simultaneous determination of these active constitutents in water-soluble R. isatidis extract. Chromatographic separation was performed on a Diamonsil C18 column (5 mm, 150 mm × 4.6 mm) with a mobile phase gradient consisting of methanol and water at a flow-rate of 1.0 mL/min, detection wavelengths 240, 250, 260 and 270 nm, the retention times of the tested five compounds were about 4.2, 5.8, 11.1, 14.2 and 20.8 min respectively, the limits of detection were 15, 12, 20, 5.8 and 24 ng/mL for cytidine, uridine, guanosine, (R,S)-goitrin and adenosine respectively, their linear ranges were between 0.045 and 350 mg/mL with correlation coefficient (R) of 0.9998-0.9999. The relative standard deviations (RSDs) of intra-day and inter-day assays were 0.30-2.36% and 0.86-2.54% respectively. Extraction recoveries were 94.25-106.21%. This novel analytical method was shown to be simple, low-cost, sensitive and reliable for multiple components in complex or undeveloped materials via MAE, ESI-MS and RODWs-HPLC.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.