Rapid Sample Pretreatment, Identification and Determination of Active Constitutents in Water‐soluble Radix isatidis Extract via MAE, ESI‐MS and RODWs‐HPLC

Abstract

In this study, we successfully studied water-soluble extract from Radix isatidis. Optimized conditions of MAE were listed, the sample can be extracted completely in 10 minutes under microwave power of 400W and solid/liquid ratio of 1:80. Active compounds in water-soluble extract from R. isatidis were identified with HPLC-DAD/ESI-MS, these compounds followed by cytidine, uridine, guanosine, (R,S)-goitrin and adenosine. RODWs-HPLC as a new sensitive chromatography were also first proposed and investigated, we favoringly used this method for simultaneous determination of these active constitutents in water-soluble R. isatidis extract. Chromatographic separation was performed on a Diamonsil C18 column (5 mm, 150 mm × 4.6 mm) with a mobile phase gradient consisting of methanol and water at a flow-rate of 1.0 mL/min, detection wavelengths 240, 250, 260 and 270 nm, the retention times of the tested five compounds were about 4.2, 5.8, 11.1, 14.2 and 20.8 min respectively, the limits of detection were 15, 12, 20, 5.8 and 24 ng/mL for cytidine, uridine, guanosine, (R,S)-goitrin and adenosine respectively, their linear ranges were between 0.045 and 350 mg/mL with correlation coefficient (R) of 0.9998-0.9999. The relative standard deviations (RSDs) of intra-day and inter-day assays were 0.30-2.36% and 0.86-2.54% respectively. Extraction recoveries were 94.25-106.21%. This novel analytical method was shown to be simple, low-cost, sensitive and reliable for multiple components in complex or undeveloped materials via MAE, ESI-MS and RODWs-HPLC.

Separation and Purification of Two Minor Compounds from Radix isatidis by Integrative MPLC and HSCCC with Preparative HPLC

“…With the distribution of ultraviolet absorption peak chromatograms and retention time, target 1 was indole-3-acetonitrile-6-O-β-D-glueosid, and target 2 was clemastanin B. [20–21] …”

A C₈-Modified Graphene@mSiO₂ Composites Based Method for Quantification of Gallic Acid in Rat Plasma after Oral Administration of Changtai Granule and Its Application to Pharmacokinetics

“…9,10) The most common and traditional procedures for TCMs are solvent extraction, Soxhlet extraction, heat-reflux extraction, and ultrasound-assisted extraction, which are most widely used currently, but these methods involve large solvent consumption or long extraction time. [11][12][13][14][15][16] New techniques like microwave-assisted extraction and supercritical fluid extraction are alternative method which can also be employed. [17][18][19][20][21] However, both of these two pretreatment techniques need high-cost specific equipment.…”