RSM-optimized IRAE sample pretreatment and HPLC simultaneous determination of Tryptanthrin, indigo, and indirubin from Chinese herbal medicineRadix Isatidis

Abstract

Summary.Radix Isatidis has widely useful activities including anti-virus, anti-bacterial. Tryptanthrin, indigo, and indirubin are active ingredients in R. Isatidis. Response surface methodology (RSM)-optimized infrared-assisted extraction (IRAE) was developed and combined with HPLC for simultaneous determination of tryptanthrin, indigo, and indirubin from R. Isatidis. IRAE were investigated through extraction yields of the three components and optimized by RSM. The optimum conditions were as follows: infrared power of 129 W, solid/liquid ratio of 1:40 g/mL, and irradiation time of 22.5 min. IRAE conditions obtained by RSM were not only accurate, but also had practical value reflecting the expected optimization. Subsequently, this novel IRAE method was evaluated by extraction yield of the components of R. Isatidis samples from different regions. Compared with common extraction methods including maceration extraction (ME), reflux extraction (RE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE), IRAE showed higher yield with advantages of no limitation of solvent selection, low cost, convenience under optimum extraction conditions. These results suggested the potential of RSM-optimized IRAE for extraction and analysis of the water-/fat-soluble compositions of Chinese herbal medicine. A simple chromatographic separation for simultaneous determination of tryptanthrin, indigo, and indirubin from Chinese herbal medicine R. Isatidis was performed on a C 18 column (Diamonsil 150 mm × 4.6 mm i.d., 5 μm) with a mobile phase isocratic consisting of methanol and water at a flow-rate of 0.8 mL min −1 . The retention times of tryptanthrin, indigo, and indirubin were 15.4, 31.9, and 58.6 min, respectively. The linear equations were obtained as follows: y = −3094.5744 + 21208.792x for tryptanthrin (R = 0.9998, 0.9-18.0 μg mL −1 ), y = 4730.0448 + 30180.567x for indigo (R = 0.9997, 0.5-10.0 μg mL −1 ) and y = −6582.9045 + 67069.312x for indirubin (R = 0.9997, 0.4-8.0 μg mL −1 ). The result showed that RSM-optimized IRAE was a simple, efficient pretreatment method for the analysis of complex matrix.

Antitumor chemical constituents of Toddalia asiatica (Linn) Lam root bark and its rational alternative medicinal parts by multivariate statistical analysis

“…About 6.0 g of root bark, root core, stem bark, stem core, near-leaf stem or leaf of T. asiatica was soaked separately in methanol for 24 h at a solid-liquid ratio of 1:50 (g:mL), extracted three times by reflux method for 2 h each time in a 65 8C-constant temperature water bath [9,11,21]. Six extracts from different parts of T. asiatica were finally prepared.…”

A C₈-Modified Graphene@mSiO₂ Composites Based Method for Quantification of Gallic Acid in Rat Plasma after Oral Administration of Changtai Granule and Its Application to Pharmacokinetics

“…9,10) The most common and traditional procedures for TCMs are solvent extraction, Soxhlet extraction, heat-reflux extraction, and ultrasound-assisted extraction, which are most widely used currently, but these methods involve large solvent consumption or long extraction time. [11][12][13][14][15][16] New techniques like microwave-assisted extraction and supercritical fluid extraction are alternative method which can also be employed. [17][18][19][20][21] However, both of these two pretreatment techniques need high-cost specific equipment.…”

Study on pharmacokinetics and tissue distribution of single dose oral tryptanthrin in Kunming mice by validated reversed-phase high-performance liquid chromatography with ultraviolet detection

“… 8 Tryptanthrin as a highly selective cyclooxygenase-2 inhibitor showed inhibitory activity on prostaglandin and leukotriene synthesis, and reduced leukotriene B4 levels with a potency comparable to that of the clinically used 5-lipoxygenase inhibitor 10 , 11 and so on. Because of its very low content in plants, less than one ten-thousandth (mass/crude drug mass), 2 , 15 and the fact that it is difficult, laborious, time-consuming, and inefficient in separation and purification of such complex natural samples, targeted synthetic methods were preferred to obtain this potential drug through microbial fermentation, 16 biomimetic synthesis 17 and chemical synthetic methods. 18 , 19 , 20 The pharmaceutical analysis method, an important tool for tryptanthrin drug development and clinical drug evaluation, can timely reflect the problems arising in the drug production process.…”