The propagation of Hypoxis rooperi S. Moore in vitro, was most efficiently achieved by initially culturing corm explants on a basal medium (BM) supplemented with 1.0 mg 1-J 6-benzylaminopurine (BA). Explants which developed shoots only on this medium, could be rooted when transferred onto a hormone-free BM. Utilizing this technique, an average of 39 plants could be obtained from a mature corm, 90% of which could be established in a sandy soil.
Three culture types of Hypoxis rooperi T. Moore were examined to determine whether hypoxoside was present. Of these cultures, only root-type cultures were found to contain hypoxoside. Quantification of this compound within these tissues using HPLC, indicated that malformed root (MR) cultures contained the highest levels of hypoxoside. In MR cultures initiated from corm explants, the hypoxoside content was found to fluctuate. Contrary to most reports, neither an increase in sucrose concentration in the basal medium (BM) nor light, stimulated hypoxoside synthesis within this tissue. Alternatively a lowering of the levels of inorganic nitrogen in the BM and the incubation of cultures in continuous darkness, enhanced hypoxoside production.
In vitro propagation of geraldton wax (Chamelaucium uncinatum Schauer) was achieved using nodal explants excised from actively growing shoots of adult plants. Murashige and Skoog basal medium (BM) supplemented with 4.4 μΜ BA was optimum for enhanced axillary shoot growth. Repeated subculturing of in vitro-grown axillary shoots resulted in a propagation rate of 5.7 ± 1.1 (±SE) shoots every 6 weeks. Sixtytwo percent of the shoots obtained by this method rooted on half-strength BM supplemented with 58.4 mM sucrose and 5.0 μΜ NAA. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), 1H-indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA).
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