Hypoxoside production in tissue cultures of Hypoxis rooperi

Page, Y. M.; Staden, J. Van

doi:10.1007/bf00044248

Cited by 11 publications

(5 citation statements)

References 19 publications

(27 reference statements)

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“…at considerably lower levels. However, as observed by Page and Van Staden in the case of H. rooperi(13), it is possible that the content of hypoxoside increases as a function of rhizome age.…”

mentioning

confidence: 77%

Plant Regeneration and Hypoxoside Content in Hypoxis obtusa

Vinesi¹,

Serafini²,

Nicoletti³

et al. 1990

J. Nat. Prod.

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Three Hypoxis species known to produce active glucosides were tested in order to assess their potential for in vitro propagation and tissue culture manipulation. Plant regeneration was achieved from the rhizome of wild type plant ofHypoxis obtusa on Murashige and Skoog medium supplemented with 1 mg/liter 6-benzylaminopurine (BAP). The other Hypoxis species tested (Hypoxis nyasica and Hypoxis angustqolia) failed to regenerate under the same culture conditions. The content and distribution of the main active principle, hypoxoside, was determined in parental and regenerated plants ofH. obtusa by hplc.

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mentioning

confidence: 77%

Plant Regeneration and Hypoxoside Content in Hypoxis obtusa

Vinesi¹,

Serafini²,

Nicoletti³

et al. 1990

J. Nat. Prod.

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“…e plant grows easily and in a wide range of environments but is extremely difficult to propagate in large quantities. A considerable amount of work has already been done on tissue culture and seed germination [94,[96][97][98][99] but without resolving a method which can supply the market with the material needed at a reasonable cost.…”

Section: Commercialisation and Conservation H Hemerocallideamentioning

confidence: 99%

Commercially Important Medicinal Plants of South Africa: A Review

Street

Prinsloo

2012

Journal of Chemistry

139

114

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ere is a growing interest in natural plant-based remedies as a source for commercial products. Around 80% of the South African population use traditional medicines to meet their primary health care needs; however, only a few South African medicinal plants have been exploited to their full potential in terms of commercialization. e opportunity for bioprospecting of plant compounds for novel pharmaceuticals remains largely untapped. Certain renowned medicinal plants of international acclaim including buchu and rooibos are currently contributing to local enterprise; however, other exciting opportunities exist for commonly used plants which have not yet reached the international arena. is paper focuses on the key research and development contributions of 10 commercially important medicinal plants of South Africa. �raditional uses, scienti�c validation, commercialisation developments, as well as both potential opportunities and setbacks are discussed.

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“…The chemical synthesis of hypoxoside, rooperol and rooperol-derivatives have been patented [10] alongside their use in the treatment of inflammation [11,12] and viral infections [13]. However, the synthetic production of hypoxoside has been documented to be difficult [14]. Moreover, the cultivation of H. hemerocallidea is known to be problematic due to lengthy seed dormancy [15], and tissue culture of H. hemerocallidea produces low yields of hypoxoside rendering these methods impractical for the extraction of certain active metabolites [14].…”

Section: Introductionmentioning

confidence: 99%

“…However, the synthetic production of hypoxoside has been documented to be difficult [14]. Moreover, the cultivation of H. hemerocallidea is known to be problematic due to lengthy seed dormancy [15], and tissue culture of H. hemerocallidea produces low yields of hypoxoside rendering these methods impractical for the extraction of certain active metabolites [14]. Research into the medicinal application of the African potato has also indicated that beneficial effects of the plant are largely dependent on the harvesting season.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and proteome of the corm, leaf and flower of Hypoxis hemerocallidea (African potato)

et al. 2021

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The corm of Hypoxis hemerocallidea, commonly known as the African potato, is used in traditional medicine to treat several medical conditions such as urinary infections, benign prostate hyperplasia, inflammatory conditions and testicular tumours. The metabolites contributing to the medicinal properties of H. hemerocallidea have been identified in several studies and, more recently, the active terpenoids of the plant were profiled. However, the biosynthetic pathways and the enzymes involved in the production of the terpene metabolites in H. hemerocallidea have not been characterised at a transcriptomic or proteomic level. In this study, total RNA extracted from the corm, leaf and flower tissues of H. hemerocallidea was sequenced on the Illumina HiSeq 2500 platform. A total of 143,549 transcripts were assembled de novo using Trinity and 107,131 transcripts were functionally annotated using the nr, GO, COG, KEGG and SWISS-PROT databases. Additionally, the proteome of the three tissues were sequenced using LC-MS/MS, revealing aspects of secondary metabolism and serving as data validation for the transcriptome. Functional annotation led to the identification of numerous terpene synthases such as nerolidol synthase, germacrene D synthase, and cycloartenol synthase amongst others. Annotations also revealed a transcript encoding the terpene synthase phytoalexin momilactone A synthase. Differential expression analysis using edgeR identified 946 transcripts differentially expressed between the three tissues and revealed that the leaf upregulates linalool synthase compared to the corm and the flower tissues. The transcriptome as well as the proteome of Hypoxis hemerocallidea presented here provide a foundation for future research.

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Hypoxoside production in tissue cultures of Hypoxis rooperi

Cited by 11 publications

References 19 publications

Plant Regeneration and Hypoxoside Content in Hypoxis obtusa

Plant Regeneration and Hypoxoside Content in Hypoxis obtusa

Commercially Important Medicinal Plants of South Africa: A Review

Transcriptome and proteome of the corm, leaf and flower of Hypoxis hemerocallidea (African potato)

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