In Vitro Propagation of Geraldton Wax: Initiation, Proliferation, and Rooting

Page, Y. M.; Visser, J.H.

doi:10.21273/hortsci.24.2.381

horts

1989

DOI: 10.21273/hortsci.24.2.381

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In Vitro Propagation of Geraldton Wax: Initiation, Proliferation, and Rooting

Y. M. Page

J.H. Visser

Abstract: In vitro propagation of geraldton wax (Chamelaucium uncinatum Schauer) was achieved using nodal explants excised from actively growing shoots of adult plants. Murashige and Skoog basal medium (BM) supplemented with 4.4 μΜ BA was optimum for enhanced axillary shoot growth. Repeated subculturing of in vitro-grown axillary shoots resulted in a propagation rate of 5.7 ± 1.1 (±SE) shoots every 6 weeks. Sixtytwo percent of the shoots obtained by this method rooted on half-strength BM supplemented with 58.4 mM sucros… Show more

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Micropropagation of some Myrtaceae species which show potential as 'new' ornamental plants

Speer¹

1993

Aust. J. Exp. Agric.

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Eleven species of Australian Myrtaceae were evaluated for their ability to be cultured in vitro. Ten species produced axillary shoots (microcuttings) suitable for inducing roots in vitro. Microcuttings of 9 species successfully developed roots and were transferred to soil culture in a glasshouse, where plants grew normally. Nodal explants were grown on a modified Murashige and Skoog (MS) medium supplemented with varying concentrations of 6-benzylaminopurine (BAP), to study shoot proliferation. Beaufortia heterophylla Turcz. explants did not respond to BAP, and all explants eventually died. The rate of shoot proliferation for the other species varied according to BAP concentration. Microcuttings of 10 species were grown on a modified half-strength MS medium supplemented with varying concentrations of the auxins indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA), to induce root formation. An increase in root number and an associated decrease in root length was observed as the concentration of IBA and NAA was increased. Verticordia muelleriana E. Pritzel did not develop roots in any treatment. Chamelaucium uncinatum Schauer cv. Purple Pride, Kunzea parvifolia Schauer, K. pulchella (Lindl.) A. S. George, Leptospermum rotundifolium (Maiden & Betche) F. Rodway ex Cheel, Verticordia drunzmondii Schauer, V. graizdis J. L. Drumm., V. hughanii F. Muell., V. nzonaclelpha Turcz., and V. roeii Endl. microcuttings developed roots both with and without added auxins. Roots that formed on microcuttings at higher auxin concentrations were generally thicker and slower in growth.

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Micropropagation of some Myrtaceae species which show potential as 'new' ornamental plants

Speer¹

1993

Aust. J. Exp. Agric.

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Effect of pH, Sucrose Concentrations and Medium States on in vitro Root-ing of Pineapple (Ananas comosus (L) Merr) cv Queen

Hamad

2021

MJSc

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The effect of medium states (liquid, semi solid, solid), pH (5.0, 5.5, 6.0, 6.5) and sucrose concentrations (10, 20, 30, 40 g/l) on in vitro rooting of pineapple cultured in full strength MS enriched with IBA at 0.5 mg/l were investigated. According to average overall sucrose concentrations, overall pH adjustments, and at each combination of equal sucrose and pH, liquid medium was always super than solid and semisolid. The tallest plantlets (66 to 71 mm) obtained in liquid medium enriched with sucrose at 10 and 20 g/l both adjusted to pH 6.0; sucrose at 20 g/l and adjusted to pH 6.5 and sucrose at 30 g/l and adjusted to pH 5.0. All of the above combinations except sucrose at 20 and pH 6.0 resulted in 100% rooting. Sucrose at 30 g/l and pH 5.0 resulted in two times more (11 roots per shoot) and three times longer roots (39 mm) than the other treatments (5 roots each about 14 mm long). Each rooting parameter had different optimum combinations of medium state, sucrose and pH adjustment. For any parameter, proper pH adjustment could reduce the optimal sucrose enrichment from 30 to 20 and even to 10 g/l. Hence, pH adjustment is suggested as an important approach for reduction of in vitro rooting medium cost.

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In Vitro Propagation of Geraldton Wax: Initiation, Proliferation, and Rooting

Cited by 2 publications

References 0 publications

Micropropagation of some Myrtaceae species which show potential as 'new' ornamental plants

Micropropagation of some Myrtaceae species which show potential as 'new' ornamental plants

Effect of pH, Sucrose Concentrations and Medium States on in vitro Root-ing of Pineapple (Ananas comosus (L) Merr) cv Queen

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