In this paper, we propose a novel scheme termed layered orthogonal frequency division multiplexing with index modulation (L-OFDM-IM) to increase the spectral efficiency (SE) of OFDM-IM systems. In L-OFDM-IM, all subcarriers are first divided into multiple layers, each determining the active subcarriers and their modulated symbols. The IM bits are carried on the indices of the active subcarriers of all layers, which are overlapped and distinguishable with different signal constellations so that the number of the IM bits is larger than that in traditional OFDM-IM. A low-complexity detection is proposed to alleviate the high burden of the optimal maximumlikelihood detection at the receiver side. A closed-form upper bound on the BER, the achievable rate and diversity order are derived to characterize the performance of L-OFDM-IM. To enhance the diversity performance of L-OFDM-IM, we further propose coordinate interleaving L-OFDM-IM (CI-L-OFDM-IM), which interleaves the real and imaginary parts of the modulated symbols over two different subchannels. Computer simulations verify the theoretical analysis, whose results show that L-OFDM-IM outperforms the conventional OFDM-IM scheme. Moreover, it is also confirmed that CI-L-OFDM-IM obtains an additional diversity order in comparison with L-OFDM-IM.

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The effects on rainbow trout, Oncorhynchus mykiss (Walbaum), immune parameters by differently formulated fish feed types containing immunostimulants have been tested in a double-blind, duplicated and controlled study performed over 50 days. A total of 800 rainbow trout (10-12 g) were kept in eight duplicate fish tanks (each containing 100 fish) and fed at a daily feeding rate of 1.5% of the biomass. The feed types were (1) control feed (C) without additives, (2) feed containing beta-glucan, nucleotides, manno-oligosaccharides (MOS), vitamins C and E (GNMCE), (3) feed containing probiotic bacteria and plant extracts (PP) and (4) feed with nucleotides, manno-oligosaccharides, vitamins C and E (NMCE). Plasma lysozyme activity was increased in fish fed two feed types (GNMCE and NMCE) but slightly depressed in fish fed PP. A non-significant trend for a higher mucous cell density at days 30 and 50 was shown in all fish receiving feeds with additives compared to the control group. All fish became infected with Ichthyophthirius multifiliis when exposed, but fish fed GNMCE showed a significantly lower infection both at days 30 and 50. Expression of genes encoding C3 and MHCII was significantly up-regulated in fish fed GNMCE for 50 days, and the expression of genes coding Hepcidin was significantly down-regulated in fish fed NMCE for 50 days. Beta-glucan was the single component, when used in combination with other feed ingredients, which was found associated with increased parasite resistance, increased lysozyme and immune gene up-regulation.

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