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Two-dimensional nanomaterials have exhibited a considerable potential for gas sensing application in recent years owing to their unique physical and chemical properties such as large surface-to-volume ratio, abundant edge sites,...

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Freeze drying has been well applied in the preparation of high-efficiency probiotic powders. However, the process is generally accompanied by probiotic viability deficiency, which is the bottleneck for further application. To improve the viability of Bifidobacterium bifidum BB01 during freeze-drying, we optimized the cryoprotectant of B. bifidum BB01 by response surface methodology (RSM) with a Central Composite Design (CCD). In this study, two values of B. bifidum BB01 with different protectant factors were investigated, including freeze-drying survival rate and the viable counts of per unit weight of freeze-dried powder. The optimized cryoprotectants were obtained as follows: glycine of 5.5%, sodium bicarbonate of 0.8%, xylo-oligosaccharides of 7%, arginine of 4.5% and skim milk of 25%. The survival rate and the viable counts of per unit weight of powder were 90.37 ± 1.9% and (2.78 ± 0.13) Â 10 11 cfuÁg À1 , respectively, both close to the predicted value (88.58% and 2.71 Â 10 11 cfuÁg À1 ). Our research demonstrated that RSM was successful in optimizing composite cryoprotectant for freezedried powder of B. bifidum which can as well protect the probiotic cells.

A Pt/MoS2/polyaniline (Pt/MoS2/PANI) nanocomposite is successfully synthesized by the hydrothermal process combined with the in situ polymerization method, and then Pt particles are decorated on its surface. The Pt/MoS2/PANI nanocomposite is deposited on a flexible Au-interdigitated electrode of a polyimide (PI) film. The flexible sensor exhibits a higher response value and fast response/recovery time to NH3 at room temperature (RT). It results in 2.32-fold and 1.13-fold improvement in the gas-sensing response toward 50 ppm NH3 compared to those of PANI and MoS2/PANI-based gas sensors. The detection limit is 250 ppb. The enhancement sensing mechanisms are attributed to the p–n heterojunction and the Schottky barrier between the three components, which has been confirmed by the current–voltage (I–V) curves. A satisfactory selectivity to NH3 against trimethylamine (TMA) and triethylamine (TEA) is obtained according to density functional theory (DFT), Bader’s analysis, and differential charge density to illustrate the adsorption behavior and charge transfer of gas molecules on the surface of the sensing materials. The sensor retains the excellent sensing response value even under high relative humidity and sensing stability at higher bending angle/numbers to NH3 gas. Hence, Pt/MoS2/PANI can be regarded as a promising sensing material for high-performance NH3 detection at room temperature applied in flexible wearable electronics.

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