Optimization of composite cryoprotectant for freeze-drying
            <i>Bifidobacterium bifidum</i>
            BB01 by response surface methodology

Chen, He; Tian, Mengqi; Chen, Li; Cui, Xiuxiu; Meng, Jiangpeng; Shu, Guowei

doi:10.1080/21691401.2019.1603157

Cited by 30 publications

(16 citation statements)

References 30 publications

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“…They used different cryoprotectants and evaluated the survival rate and viable cell numbers per unit weight of the resulting freeze-dried powder. The results suggested that the best cryoprotectant for B. bifidum was xylooligosaccharides with the survival rate and the viable cell numbers per unit weight of powder were around 90% and 11 logs, respectively (Chen et al, 2019). Another study using freezedrying with Bifidobacterium spp.…”

Section: Drying Processesmentioning

confidence: 99%

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

Andrade¹,

Almeida²,

Domingos³

et al. 2020

Front. Bioeng. Biotechnol.

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In the last years several human commensals have emerged from the gut microbiota studies as potential probiotics or therapeutic agents. Strains of human gut inhabitants such as Akkermansia, Bacteroides, or Faecalibacterium have shown several interesting bioactivities and are thus currently being considered as food supplements or as live biotherapeutics, as is already the case with other human commensals such as bifidobacteria. The large-scale use of these bacteria will pose many challenges and drawbacks mainly because they are quite sensitive to oxygen and/or very difficult to cultivate. This review highlights the properties of some of the most promising human commensals bacteria and summarizes the most up-to-date knowledge on their potential health effects. A comprehensive outlook on the potential strategies currently employed and/or available to produce, stabilize, and deliver these microorganisms is also presented.

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Section: Drying Processesmentioning

confidence: 99%

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

Andrade¹,

Almeida²,

Domingos³

et al. 2020

Front. Bioeng. Biotechnol.

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“…Especially compounds stabilizing intra-cellular structures must be absorbed by the cells before the freezing process begins. For example, skim milk is not absorbed by microorganisms but forms a protective layer on the cell surface whereas other protectants may permeate the cell wall or cell membrane [22]. This applies to sugars such as trehalose, maltose or lactose, which in general enhance the glass transition temperature, thereby restricting the molecular mobility and thus stabilizing the cells during freeze-drying [23,24].…”

Section: Cryoprotectantsmentioning

confidence: 99%

Along the Process Chain to Probiotic Tablets: Evaluation of Mechanical Impacts on Microbial Viability

et al. 2020

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Today, probiotics are predominantly used in liquid or semi-solid functionalized foods, showing a rapid loss of cell viability. Due to the increasing spread of antibiotic resistance, probiotics are promising in pharmaceutical development because of their antimicrobial effects. This increases the formulation requirements, e.g., the need for an enhanced shelf life that is achieved by drying, mainly by lyophilization. For oral administration, the process chain for production of tablets containing microorganisms is of high interest and, thus, was investigated in this study. Lyophilization as an initial process step showed low cell survival of only 12.8%. However, the addition of cryoprotectants enabled survival rates up to 42.9%. Subsequently, the dried cells were gently milled. This powder was tableted directly or after mixing with excipients microcrystalline cellulose, dicalcium phosphate or lactose. Survival rates during tableting varied between 1.4% and 24.1%, depending on the formulation and the applied compaction stress. More detailed analysis of the tablet properties showed advantages of excipients in respect of cell survival and tablet mechanical strength. Maximum overall survival rate along the complete manufacturing process was >5%, enabling doses of 6 × 10 8 colony forming units per gram ( CFU g total − 1 ), including cryoprotectants and excipients.

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“…Skim milk (20% w/v) provided the highest protection for cell survival at 89.26% followed by lactose (10% w/v) with 87.78% survival rate. Skim milk is one of the most preferable protective agents and widely studied for freeze-drying of various types of bacteria [16,[28][29][30]. Proteins and calcium in the skim milk may contribute to cell protection from the extreme and harsh conditions of the freeze-drying process by forming a protective coating on the cell wall [31].…”

Section: Selection Of Protective Agents For Bioformulation Of P Polymentioning

confidence: 99%

“…In this study, the considered cell viability of P. polymyxa Kp10 after freeze-drying was mainly affected by types and concentrations of the protective agent. To determine the optimal protective agent combination, the ranges of skim milk concentration (10%-30% w/v), lactose (5%-15% w/v), and sucrose (15%-40% w/v) were chosen based on the previous preliminary test and several other reported studies [11,28,30,40].…”

Section: Experimental Design For Optimization Of Protective Agent Commentioning

confidence: 99%

Optimization of Protective Agents for The Freeze-Drying of Paenibacillus polymyxa Kp10 as a Potential Biofungicide

2020

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Anthracnose is a fungal disease causing major losses in crop production. Chemical fungicides widely used in crop plantations to combat fungal infections can be a threat to the environment and humans in the long term. Recently, biofungicides have gained much interest as an alternative to chemical fungicides due to their environmentally friendly nature. Biofungicide products in powder form can be formulated using the freeze-drying technique to provide convenient storage. Protective agent formulation is needed in maintaining the optimal viable cells of biofungicide products. In this study, 8.10 log colony-forming unit (CFU)/mL was the highest cell viability of Paenibacillus polymyxa Kp10 at 22 h during incubation. The effects of several selected protective agents on the viability of P. polymyxa Kp10 after freeze-drying were studied. Response surface methodology (RSM) was used for optimizing formulation for the protective agents. The combination of lactose (10% w/v), skim milk (20% w/v), and sucrose (27.5% w/v) was found to be suitable for preserving P. polymyxa Kp10 during freeze-drying. Further, P. polymyxa Kp10 demonstrated the ability to inhibit fungal pathogens, Colletotrichum truncatum and C. gloeosporioides, at 60.18% and 66.52% of inhibition of radial growth, respectively.

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Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology

Cited by 30 publications

References 30 publications

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

Along the Process Chain to Probiotic Tablets: Evaluation of Mechanical Impacts on Microbial Viability

Optimization of Protective Agents for The Freeze-Drying of Paenibacillus polymyxa Kp10 as a Potential Biofungicide

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