Optimization of Protective Agents for The Freeze-Drying of Paenibacillus polymyxa Kp10 as a Potential Biofungicide

Abstract

Anthracnose is a fungal disease causing major losses in crop production. Chemical fungicides widely used in crop plantations to combat fungal infections can be a threat to the environment and humans in the long term. Recently, biofungicides have gained much interest as an alternative to chemical fungicides due to their environmentally friendly nature. Biofungicide products in powder form can be formulated using the freeze-drying technique to provide convenient storage. Protective agent formulation is needed in maintaining the optimal viable cells of biofungicide products. In this study, 8.10 log colony-forming unit (CFU)/mL was the highest cell viability of Paenibacillus polymyxa Kp10 at 22 h during incubation. The effects of several selected protective agents on the viability of P. polymyxa Kp10 after freeze-drying were studied. Response surface methodology (RSM) was used for optimizing formulation for the protective agents. The combination of lactose (10% w/v), skim milk (20% w/v), and sucrose (27.5% w/v) was found to be suitable for preserving P. polymyxa Kp10 during freeze-drying. Further, P. polymyxa Kp10 demonstrated the ability to inhibit fungal pathogens, Colletotrichum truncatum and C. gloeosporioides, at 60.18% and 66.52% of inhibition of radial growth, respectively.

Using combined optimization and vacuum freeze drying technology to prepare directed vat set starter for “Niuganba,” a fermented beef

“…The response surface design method is an effective method to establish the correlation between multiple variables. As demonstrated in the previous studies, the response surface design method could successfully optimize the freeze-dried powder formulation (Nasran et al, 2020;Shu et al, 2019). Directed vat set starter is a highly concentrated and standardized starter strain with high fermentation efficiency.…”

Lyophilization process optimization and molecular dynamics simulation of mRNA-LNPs for SARS-CoV-2 vaccine

“…However, drying of mRNA-LNPs is more sophisticated as the freezing and the dehydration process induces mechanical force and deforms the vehicle structure, leading to vehicle aggregation, mRNA breakage, or leakage 24,25 . Some studies have shown that although mRNA-LNPs maintain their integrity and encapsulation efficiency, the in vivo uptake efficacy is greatly reduced after lyophilization due to some unknown reasons 15,[26][27][28][29] . In addition, the reported studies regarding mRNA-LNPs lyophilization mostly focus on the maintenance of its biological activity, while there are few studies on the lyophilization procedures 30 .…”

Effectiveness of nutrient media for the recovery of lyophilisates of fastidious microorganisms: evidence from Streptococcus spp.

“…They are not resistant to the influence of physical environmental factors, die quickly, and are demanding to the cultivation conditions. Therewith, if cultivation and storage conditions are not observed, bacteria lose their virulence, which reduces their scientific value for researchers and practical use (Nasran et al, 2020).…”