Effects of stachyose on intestinal microbiota and immunity in mice infected with enterotoxigenic Escherichia coli

Xi, Menglu; Yao, Qi; Ge, Wupeng; Chen, Ying; Cao, Binyun; Wang, Zhongfu; Cui, Xiuxiu; Sun, Qiwu

doi:10.1016/j.jff.2019.103689

Cited by 15 publications

(20 citation statements)

References 44 publications

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“…The differences in immunomodulatory property by L. casei between the two studies are probably due to different L. casei strains were used. This is supported by the findings that Lactobacillus plantarum K55-5 and L. plantarum K8 isolated from different sources exert different effects on immune response in mouse (Lee et al, 2016), demonstrating the specificity of different Lactobacillus strains, while the stachyose-associated immune regulation probably involved improvement of intestinal microbiota composition, activation of immune cells and production of short chain fat acid (Shang et al, 2020;Xi et al, 2020).…”

Section: Assessment Of Microbiota Of DI Mucosasupporting

confidence: 55%

Dietary supplementation of stachyose and Lactobacillus casei improves the immunity and intestinal health of turbot ( Scophthalmus maximus . L)

Dai

et al. 2021

Aquaculture Nutrition

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A 12-week feeding trial was conducted to investigate the effects of dietary Lactobacillus casei, stachyose, or combined (synbiotic) on immunity and intestinal health of turbot (Scophthalmus maximus. L). Four isonitrogenous and isolipidic diets were formulated: Control diet, Control diet with 1.0×10 8 CFU/g L. casei (LC), 25.0 g kg -1 stachyose (ST) or 1.0 × 10 8 CFU/g L. casei + 25.0 g kg -1 stachyose (SL) supplementation separately.The results showed that fish-fed diets ST and SL had a significant higher activity of lysozyme and concentration of complement 3 and 4, and IgM in serum, while the higher activity of ACP was observed in LC and SL group. The highest viable counts of L. casei in mucosa and content of DI were observed in SL group. Diet SL significantly increased the PR of DI, expression of tight junction proteins, pcna and the bcl-2/bax, while decreased the expression of caspase-3 and caspase-9. Moreover, diets LC, ST and SL remarkably decreased the abundance of Mycoplasma but increased the abundance of Lactobacillus and Bacteroides, α-diversity, and altered the bacterial communities in DI. Therefore, both stachyose and L. casei could enhance the immunity and intestinal health of turbot, while the synbiotic was superior to individual administration of stachyose or L. casei.

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Section: Assessment Of Microbiota Of DI Mucosasupporting

confidence: 55%

Dietary supplementation of stachyose and Lactobacillus casei improves the immunity and intestinal health of turbot ( Scophthalmus maximus . L)

Dai

et al. 2021

Aquaculture Nutrition

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“…After a lethal challenge with ETEC H10407, the most remarkable alterations, including severe villi loss and necrosis, congestion with haemorrhage, and neutrophil infiltration, were observed in the intestine of unimmunised mice. Similar findings have been supported by a number of earlier investigations [42,49,50]. Notably, the immunised mice with MyChol™ did not show any mortality, diarrhoeal symptoms, and loss of body weight for 14 days after the ETEC challenge.…”

Section: Discussionsupporting

confidence: 89%

Dual-Use Vaccine for Diarrhoeal Diseases: Cross-Protective Immunogenicity of a Cold-Chain-Free, Live-Attenuated, Oral Cholera Vaccine against Enterotoxigenic Escherichia coli (ETEC) Challenge in BALB/c Mice

et al. 2022

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In low- and middle-income countries, diarrhoeal diseases are the second most common cause of mortality in children, mainly caused by enterotoxin-producing bacteria, such as Shigella, Vibrio, Salmonella, and Escherichia coli. Cholera and traveller’s diarrhoea are caused by Vibrio cholerae (O1 and O139 serogroups) and enterotoxigenic Escherichia coli (ETEC), respectively. The cholera toxin (CT) produced by V. cholerae and the heat-labile enterotoxin (LT) of ETEC are closely related by structure, function, and the immunological response to them. There is no exclusive vaccine for ETEC; however, cholera vaccines based on the CT-B component elicit a short-term cross-protection against ETEC infection. In this context, the cross-protective efficacy of MyCholTM, a prototype cold-chain-free, live-attenuated, oral cholera vaccine against V. cholerae O139 was evaluated in BALB/c mice. The 100% lethal dose (LD100) of 109 CFU/mL of the ETEC H10407 strain was used for the challenge studies. The mice immunised with MyChol™ survived the challenge by producing anti-CT antibodies, which cross-neutralised the LT toxin with no body weight loss and no sign of diarrhoea. Compared to unimmunised mice, the immunised mice elicited the neutralising antitoxin that markedly decreased ETEC colonisation and fluid accumulation caused by ETEC H10407 in the intestines. The immunised mice recorded higher antibody titres, including anti-CT IgG, anti-LT IgG, anti-CT-B IgG, and anti-LTB IgG. Only a two-fold rise in anti-CT/CT-B/LT/LT-B IgA was recorded in serum samples from immunised mice. No bactericidal antibodies against ETEC H10407 were detected. This investigation demonstrates the safety, immunogenicity, and cross-protective efficacy of MyCholTM against the ETEC H10407 challenge in BALB/c mice.

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“…SCFAs (acetic acid, propionic acid, butyric acid, and valeric acid) were tested by gas chromatography (GC) as described previously . Briefly, 0.100 ± 0.010 g of the intestinal contents was weighed accurately into the stool sample box and processed with the stool processor (HALO-F100, Hailu Biotechnology Inc., China) to prepare a 10% suspension using Milli-Q water.…”

Section: Methodsmentioning

confidence: 99%

“…SCFAs (acetic acid, propionic acid, butyric acid, and valeric acid) were tested by gas chromatography (GC) as described previously. 34 Briefly, 0.100 ± 0.010 g of the intestinal contents was weighed accurately into the stool sample box and processed with the stool processor (HALO-F100, Hailu Biotechnology Inc., China) to prepare a 10% suspension using Milli-Q water. 0.5 mL of the suspension was pipetted in a 1.5 mL centrifuge tube, to which 100 μL of 25% (w/v) crotonic acid (internal standard) and metaphosphoric acid was added and frozen at −30 °C for 24 h. The suspension was centrifuged at 5000g for 3 min at 4 °C to remove impurities after thawing.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Bifidobacterium bifidum E3 Combined with Bifidobacterium longum subsp. infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo

Yue

Wang²,

Xie

et al. 2023

J. Agric. Food Chem.

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Changes in the functions of the intestinal barrier occur in parallel with the development of sepsis. The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS). The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells. The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group. In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α). The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids. Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate. This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota. Bifidobacterium bifidum E3 combined with Bifidobacterium longum subsp. infantis E4 may thus serve as a probiotic against the intestinal injury caused by LPS.

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Effects of stachyose on intestinal microbiota and immunity in mice infected with enterotoxigenic Escherichia coli

Cited by 15 publications

References 44 publications

Dietary supplementation of stachyose and Lactobacillus casei improves the immunity and intestinal health of turbot ( Scophthalmus maximus . L)

Dietary supplementation of stachyose and Lactobacillus casei improves the immunity and intestinal health of turbot ( Scophthalmus maximus . L)

Dual-Use Vaccine for Diarrhoeal Diseases: Cross-Protective Immunogenicity of a Cold-Chain-Free, Live-Attenuated, Oral Cholera Vaccine against Enterotoxigenic Escherichia coli (ETEC) Challenge in BALB/c Mice

Bifidobacterium bifidum E3 Combined with Bifidobacterium longum subsp. infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo

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