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Highlights d We examine the sufficiency of the ''no net loss'' goal for managing China's wetlands d China's wetlands showed a net increase of 1,548 km 2 between 2000 and 2015 d Conservation efforts to restore wetlands did not offset human-caused wetland losses d The ''no net loss'' goal should be used carefully as a target for wetland conservation

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Nitrogen-doped CDs (N-CDs) were firstly prepared by using pear juice as the carbon source and ethanediamine as a nitrogen doping precursor with a microwave assisted pyrolysis technique. Based on the fluorescence recovery induced by competitive adsorption and desorption, a label-free “turn on” fluorescence assay with high sensitivity and selectivity was proposed for the analysis of CA125.

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Gold nanoclusters (Au NCs) coated with various peptides have been widely used as fluorescent probes, and nowadays the most commonly used are cysteine (C) and tyrosine (Y) based ones. Herein, we report the preparation and clinical application of highly efficient and stable fluorescent Au NCs protected by screened peptides with a specific amino acid sequence Cys-Met-Met-Met-Met-Met (CMMMMM). Compared with traditional C, Y based peptide (CYYYYY) protected Au NCs, the fluorescence intensity of the CMMMMM-Au NCs increased by 230%, and the photobleaching resistance or stability of the CMMMMM-Au NCs increased by about 300% (after continuous ultraviolet irradiation for 60 min, the fluorescence of the CMMMMM-Au NCs remained more than 90% of their initial intensity, while the CYYYYY-Au NCs remained less than 30%). Assaying arrays based on CMMMMM protected Au NCs with different positive or negative charges as sensing receptors were developed through regulating different pH values, and multivariate analysis on the patterns obtained by these arrays allowed effective identification of not only ten proteins separately but also complex protein mixtures with subtly diverse compositions. The docking simulation and isothermal titration confirmed that target proteins interacted with CMMMMM-Au NCs mainly through electrostatic interactions and partly hydrophobic interactions, which affected the binding energy and fluorescence lifetime of CMMMMM-Au NCs, resulting in the unique fingerprint-like recognition patterns. Furthermore, serums from breast cancer, severe osteoarthritis, and rectal cancer patients can be effectively identified with healthy people using this CMMMMM-Au NCs based sensor array.

This study discusses generalized Rayleigh quotient and high efficiency finite element discretization schemes. Some results are as follows: 1) Rayleigh quotient accelerate technique is extended to nonselfadjoint problems. Generalized Rayleigh quotients of operator form and weak form are defined and the basic relationship between approximate eigenfunction and its generalized Rayleigh quotient is established. 2) New error estimates are obtained by replacing the ascent of exact eigenvalue with the ascent of finite element approximate eigenvalue. 3) Based on the work of Xu Jinchao and Zhou Aihui, finite element two-grid discretization schemes are established to solve nonselfadjoint elliptic differential operator eigenvalue problems and these schemes are used in both conforming finite element and nonconforming finite element. Besides, the efficiency of the schemes is proved by both theoretical analysis and numerical experiments. 4) Iterated Galerkin method, interpolated correction method and gradient recovery for selfadjoint elliptic differential operator eigenvalue problems are extended to nonselfadjoint elliptic differential operator eigenvalue problems.

Schematic illustration of Pdots produced by FNP.