The inflammatory system activated by uterine infection is associated with decreased fertility. Diseases can be detected in advance by identifying biomarkers of several uterine diseases. Escherichia coli is one of the most frequent bacteria that is involved in pathogenic processes in dairy goats. The purpose of this study was to investigate the effect of endotoxin on protein expression in goat endometrial epithelial cells. In this study, the LC–MS/MS approach was employed to investigate the proteome profile of goat endometrial epithelial cells. A total of 1180 proteins were identified in the goat Endometrial Epithelial Cells and LPS-treated goat Endometrial Epithelial Cell groups, of which, 313 differentially expressed proteins were accurately screened. The proteomic results were independently verified by WB, TEM and IF techniques, and the same conclusion was obtained. To conclude, this model is suitable for the further study of infertility caused by endometrial damage caused by endotoxin. These findings may provide useful information for the prevention and treatment of endometritis.
Xinong Saanen goats are among the most common dairy goats in China and serve as major sources of milk in the Chinese dairy industry. Escherichia coli is a Gram-negative bacterium frequently implicated in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria and is involved in pathogenic processes that lead to post-partum metritis and endometritis in dairy goats. After binding to toll-like receptor 4, LPS induces reactions in the endometrium, leading to acute or chronic inflammation, which impairs reproductive function. The purpose of this study was to evaluate the effects of LPS on goat endometrial epithelial cells (gEECs) and the changes that occur in response to LPS. The proteomic map of gEECs exposed to different concentrations of LPS was studied using unlabeled liquid chromatography with tandem mass spectrometry. A total of 1178 proteins were identified in all samples, and 13 differentially expressed proteins were subjected to quantitative and gene ontology functional annotation analyses. In conclusion, this study shows that specific proteins expressed in the goat uterus are altered according to the concentration of LPS.
Repeat breeding is considered to be one of the economically important reproductive disorders in cattle, as it results in an increased number of inseminations and associated costs, and an increase in the number of days open. The physiological cause of the repeat breeding syndrome is complex and multifactorial. Uterine luminal fluid from the health and repeat breeder groups was analyzed in a holistic proteomic approach using LC-MS/MS analysis combined with a label-free quantification approach. In total, 882 proteins were identified, of which 154 differed significantly in abundance between the two groups. Evaluation of this dataset using bioinformatic tools revealed an assignment of higher abundant proteins in repeat breeder group to several pathway processes, such as carbon metabolism, purine metabolism and biosynthesis of amino acids. We used proteomic methods based on gel mass spectrometry to characterize the changes of proteomic characteristics associated with uterine diseases. These findings provide valuable information towards the identification of critical biomarker proteins for repeat breeding syndrome in cattle.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.