A Comparative Proteomic Analysis to Explore the Influencing Factors on Endometritis Using LC-MS/MS

Abstract

The inflammatory system activated by uterine infection is associated with decreased fertility. Diseases can be detected in advance by identifying biomarkers of several uterine diseases. Escherichia coli is one of the most frequent bacteria that is involved in pathogenic processes in dairy goats. The purpose of this study was to investigate the effect of endotoxin on protein expression in goat endometrial epithelial cells. In this study, the LC–MS/MS approach was employed to investigate the proteome profile of goat endometrial epithelial cells. A total of 1180 proteins were identified in the goat Endometrial Epithelial Cells and LPS-treated goat Endometrial Epithelial Cell groups, of which, 313 differentially expressed proteins were accurately screened. The proteomic results were independently verified by WB, TEM and IF techniques, and the same conclusion was obtained. To conclude, this model is suitable for the further study of infertility caused by endometrial damage caused by endotoxin. These findings may provide useful information for the prevention and treatment of endometritis.

Investigation of Uterine Fluid Extracellular Vesicles’ Proteomic Profiles Provides Novel Diagnostic Biomarkers of Bovine Endometritis

“…Conversely, another research study has identified 186 proteins showing differential enrichment in UF between healthy cows and those with SE [ 70 ]. Moreover, a separate investigation revealed 330 proteins exhibiting differential enrichment in cell cultures between the group treated with lipopolysaccharide (LPS), an endotoxin recognized for inducing uterine inflammation, and the non-treated group [ 30 ]. Variations in the quantity/number of differentially enriched proteins may result from variances in the materials analysed, disparities in MS techniques, and differences in the methodology used to classify the SE.…”

“…In cattle, EVs have also been researched as a potential biomarker to detect and monitor diseases in herds [ 26 , 27 , 28 ]. Some EV studies have induced uterine infection resulting in endometritis in vivo [ 26 ] or replicated the uterine conditions in cell culture [ 29 , 30 ]. These investigations have revealed variations in the miRNA or proteomic composition of EVs between healthy and inflamed conditions.…”