Proteomic analysis of uterine lavage fluid of dairy cows at different time after delivery by mass spectrometry

Jiang, Xingcan; Li, Ziyuan; Chang, Xiaofeng; Cong, Hongliang; Qiu, Rendong; Wang, Aihua; Lin, Peng; Tang, Kai-Fu; Chen, Huatao; Zhang, Dong; Jin, Yaping

doi:10.1016/j.theriogenology.2023.05.013

Cited by 2 publications

(3 citation statements)

References 37 publications

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“…Nonetheless, our study identified HTRA1 as a significantly expressed protein in SE samples compared to both healthy cows and cows with CLE. Interestingly, similarly have been noted the distinct enrichment of HTRA1 in the LPS-treated group compared to the control, which is consistent with our observations [ 70 ]. Therefore, HTRA1 emerges as a promising biomarker for SE, facilitating differentiation between CLE and healthy cows.…”

Section: Discussionsupporting

confidence: 93%

“…In our study, we observed 17 proteins in UF-EVs that showed varying levels of enrichment between healthy cows and those with SE. Conversely, another research study has identified 186 proteins showing differential enrichment in UF between healthy cows and those with SE [ 70 ]. Moreover, a separate investigation revealed 330 proteins exhibiting differential enrichment in cell cultures between the group treated with lipopolysaccharide (LPS), an endotoxin recognized for inducing uterine inflammation, and the non-treated group [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

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Investigation of Uterine Fluid Extracellular Vesicles’ Proteomic Profiles Provides Novel Diagnostic Biomarkers of Bovine Endometritis

Piibor,

Waldmann,

Prasadani

et al. 2024

Biomolecules

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Cow uterine infections pose a challenge in dairy farming, resulting in reproductive disorders. Uterine fluid extracellular vesicles (UF-EVs) play a key role in cell-to-cell communication in the uterus, potentially holding the signs of aetiology for endometritis. We used mass spectrometry-based quantitative shotgun proteomics to compare UF-EV proteomic profiles in healthy cows (H), cows with subclinical (SE) or clinical endometritis (CLE) sampled at 28–35 days postpartum. Functional analysis was performed on embryo cultures with the exposure to different EV types. A total of 248 UF-EV proteins exhibited differential enrichment between the groups. Interestingly, in SE, EV protein signature suggests a slight suppression of inflammatory response compared to CLE-UF-EVs, clustering closer with healthy cows’ profile. Furthermore, CLE-UF-EVs proteomic profile highlighted pathways associated with cell apoptosis and active inflammation aimed at pathogen elimination. In SE-UF-EVs, the regulation of normal physiological status was aberrant, showing cell damage and endometrial repair at the same time. Serine peptidase HtrA1 (HTRA1) emerged as a potential biomarker for SE. Supplementation of CLE- and SE-derived UF-EVs reduced the embryo developmental rates and quality. Therefore, further research is warranted to elucidate the precise aetiology of SE in cattle, and HTRA1 should be further explored as a potential diagnostic biomarker.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Investigation of Uterine Fluid Extracellular Vesicles’ Proteomic Profiles Provides Novel Diagnostic Biomarkers of Bovine Endometritis

Piibor,

Waldmann,

Prasadani

et al. 2024

Biomolecules

View full text Add to dashboard Cite

show abstract

“…Bovine endometrial epithelial cells (bEECs), from a cell line maintained in our laboratory [ 22 ], were thawed and cultured in 6-well plates in a medium comprising 90% high-glucose Dulbecco’s modified Eagle’s medium (DMEM, BL304A, Biosharp, Guangzhou, China) and 10% fetal bovine serum (FBS, Z7186FBS-500, ZETA LIFE, San Francisco, CA, USA) with 37 °C in a 5% CO 2 and atmospheric air environment with saturation humidity. To determine the optimal time and concentration of cecropin A, we conducted initial experiments using the CCK-8 assay.…”

Section: Methodsmentioning

confidence: 99%

Cecropin A Alleviates LPS-Induced Oxidative Stress and Apoptosis of Bovine Endometrial Epithelial Cells

Zhao,

Zhang,

Sun

et al. 2024

Animals

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Dairy cows receiving a prolonged high-concentrate diet express an elevated concentration of lipopolysaccharides (LPSs) in the peripheral blood circulation, accompanied by a series of systemic inflammatory responses; however, the specific impacts of inflammation are yet to be determined. Cecropin-like antimicrobial peptides have become a research hotspot regarding antimicrobial peptides because of their excellent anti-inflammatory activities, and cecropin A is a major member of the cecropin family. To elucidate the mechanism of cecropin A as anti-inflammatory under the condition of sub-acute ruminal acidosis (SARA) in dairy cows, we induced inflammation in bEECs with LPS (10 µg/mL) and then added cecropin A (25 µM). Afterwards, we detected three categories of indexes including oxidative stress indices, inflammation-related genes, and apoptosis-related genes in bovine endometrial epithelial cells (bEECs). The results indicated that cecropin A has the ability to reduce inflammatory factors TNF-α, IL-1β, and IL-8 and inhibit the MAPK pathway to alleviate inflammation. In addition, cecropin A is able to reduce reactive oxygen species (ROS) levels and alleviates LPS-induced oxidative stress and mitochondrial dysfunction by downregulating NADPH Oxidase (NOX), and upregulating catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD). Furthermore, cecropin A demonstrates the ability to inhibit apoptosis by suppressing the mitochondrial-dependent apoptotic pathway, specifically Fas/FasL-caspase-8/-3. The observed increase in the Bcl-2/Bax ratio, a known apoptosis regulator, further supports this finding. In conclusion, our study presents novel solutions for addressing inflammatory responses associated with SARA.

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Proteomic analysis of uterine lavage fluid of dairy cows at different time after delivery by mass spectrometry

Cited by 2 publications

References 37 publications

Investigation of Uterine Fluid Extracellular Vesicles’ Proteomic Profiles Provides Novel Diagnostic Biomarkers of Bovine Endometritis

Investigation of Uterine Fluid Extracellular Vesicles’ Proteomic Profiles Provides Novel Diagnostic Biomarkers of Bovine Endometritis

Cecropin A Alleviates LPS-Induced Oxidative Stress and Apoptosis of Bovine Endometrial Epithelial Cells

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