Functional constipation (FC) is one of the most common gastrointestinal disorders characterized by hard stools and infrequent bowel movements, which is associated with dysfunction of the enteric nervous system and intestinal motility. Luteolin, a naturally occurring flavone, was reported to possess potential pharmacological activities on intestinal inflammation and nerve injury. This study aimed to explore the role of luteolin and its functional mechanism in loperamide-induced FC mice. Our results showed that luteolin treatment reversed the reduction in defecation frequency, fecal water content, and intestinal transit ratio, and the elevation in transit time of FC models. Consistently, luteolin increased the thickness of the muscular layer and lessened colonic histopathological injury induced by loperamide. Furthermore, we revealed that luteolin treatment increased the expression of neuronal protein HuC/D and the levels of intestinal motility-related biomarkers, including substance P (SP), vasoactive intestinal polypeptide (VIP), and acetylcholine (ACh), as well as interstitial cells of Cajal (ICC) biomarker KIT proto-oncogene, receptor tyrosine kinase (C-Kit), and anoctamin-1 (ANO1), implying that luteolin mediated enhancement of colonic function and contributed to the anti-intestinal dysmotility against loperamide-induced FC. Additionally, luteolin decreased the upregulation of aquaporin (AQP)-3, AQP-4, and AQP-8 in the colon of FC mice. In summary, our data showed that luteolin might be an attractive option for developing FC-relieving medications.
Two new highly-oxygenated neo-clerodane diterpenoids, 3S-acetoxyl-mollotucin D dilactone ester (1) and 6S-crotoeurin C (2), and a new lupane-type triterpene, 16β-hydroxyl-3β-O-trans-coumaroyl-betulin (6), as well as three known analogues (3−5) were obtained from the leaves of Croton laui. The structures of the new compounds were determined by extensive spectroscopic methods, and their absolute configurations were determined by combination of single-crystal X-ray diffraction analysis, electronic circular dichroism (ECD) spectra, and literature data. Compounds 2 and 3 exhibited inhibitory activities of lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages with IC 50 values of 1.2 and 1.6 μM, respectively. Additionally, compound 6 exhibited activity against Col205 and HepG2 cell lines with IC 50 values of 12.9 and 17.7 μM, respectively.
Functional constipation (FC), a common symptom that is primarily associated with intestinal motility dysfunction, is a common problem worldwide. Arctiin (Arc) is a lignan glycoside isolated from the Chinese herbal medicine Arctium lappa L., which is a health food in China.
With the rapid development of society, women have to bear multiple pressures from family, life and work, which virtually affect the function of ovarian reserve. In order to explore the relationship between emotional factors and ovarian reserve function, by analyzing the importance and harmfulness of emotional factors to ovarian reserve function, this paper highlights the need to soothe the liver and regulate qi in the whole process of treatment. The importance of emotional factors to ovarian reserve function is emphasized, and it is concluded that there is a significant clinical effect in the treatment of ovarian reserve function from the liver theory, and it is advocated that clinical attention should be paid to the regulating effect of emotion on ovarian reserve function. In order to achieve the purpose of harmony of qi and blood, at the same time, this paper is expected to provide a certain scientific basis for clinical treatment. Another case 1 is attached for corroboration.
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