To avoid the defocusing effects of propagating waves through salt and overburden with an inaccurate overburden velocity model, we introduce a vertical seismic profiling ͑VSP͒ local elastic reverse-time-migration ͑RTM͒ method for salt-flank imaging by transmitted P-to-S waves. This method back-projects the transmitted PS waves using a local velocity model around the well until they are in phase with the back-projected PP waves at the salt boundaries. The merits of this method are that it does not require the complex overburden and salt-body velocities and it automatically accounts for source-side statics. In addition, the method accounts for kinematic and dynamic effects, including anisotropy, absorption, and all other unknown rock effects outside of this local subsalt velocity model. Numerical tests on an elastic salt model and offset 2D VSP data in the Gulf of Mexico, using a finite-difference time-domain staggered-grid RTM scheme, partly demonstrate the effectiveness of this method over interferometry PS-PP transmission migration and local acoustic RTM. Our method separates elastic wavefields to vector P-and S-wave velocity components at the trial image point and achieves better resolution than local acoustic RTM and interferometric transmission migration. The analytical formulas of migration resolution for local acoustic and elastic RTM show that the migration illumination is limited by data frequency and receiver aperture, and the spatial resolution is lower than standard poststack and prestack migration. This new method can image salt flanks as well as subsalt reflectors.

DNA methylation disturbance is associated with defective human sperm. However, oligozoospermia (OZ) and asthenozoospermia (AZ) usually present together, and the relationship between the single-phenotype defects in human sperm and DNA methylation is poorly understood. In this study, 20 infertile OZ patients and 20 infertile AZ patients were compared with 20 fertile normozoospermic men. Bisulfate-specific PCR was used to analyze DNA methylation of the H19-DMR and the DAZL promoter in these subjects. A similar DNA methylation pattern of the H19-DMR was detected in AZ and NZ(control), with only complete methylation and mild hypomethylation(<50% unmethylated CpGs) identified, and there was no significant difference in the occurrence of these two methylation patterns between AZ and NZ (P>0.05). However, the methylation pattern of severe hypomethylation (>50% unmethylated CpGs ) and complete unmethylation was only detected in 5 OZ patients, and the occurrence of these two methylation patterns was 8.54±10.86% and 9±6.06%, respectively. Loss of DNA methylation of the H19-DMR in the OZ patients was found to mainly occur in CTCF-binding site 6, with occurrence of 18.15±14.71%, which was much higher than that in patients with NZ (0.84±2.05%) and AZ (0.58±1.77%) (P<0.001).Additional, our data indicated the occurrence of >20% methylated clones in the DAZL promoter only in infertile patients, there was no significant difference between the AZ and OZ patients in the proportion of moderately-to-severely hypermethylated clones (p>0.05). In all cases, global sperm genome methylation analyses, using LINE1 transposon as the indicator, showed that dysregulation of DNA methylation is specifically associated with the H19-DMR and DAZL promoter. Therefore, abnormal DNA methylation status of H19-DMR, especially at the CTCF-binding site 6, is closely associated with OZ. Abnormal DNA methylation of the DAZL promoter might represent an epigenetic marker of male infertility.

Collectively, SA3K protects against oxidative stress by targeting the ROS generation/degradation system and modulating the KEAP1-NRF2 signaling pathway.

An approach is described for creating random complex screens to be used in computer simulations of arbitrary Schell-model beams with a prescribed far-field intensity distribution. Simulation examples including beam profiles with reflection symmetry and rotational symmetry, flat-top, and pyramidal shapes are presented to verify the proposed approach. A more general scenario with a nonsymmetric far-field beam shape is illustrated to demonstrate the evolution in the free-space propagation from the source plane to the far zone.

A technique is presented to produce any desired partially coherent Schell-model source using a single phase-only liquid-crystal spatial light modulator (SLM). Existing methods use SLMs in combination with amplitude filters to manipulate the phase and amplitude of an initially coherent source. The technique presented here controls both the phase and amplitude using a single SLM, thereby making the amplitude filters unnecessary. This simplifies the optical setup and significantly increases the utility and flexibility of the resulting system. The analytical development of the technique is presented and discussed. To validate the proposed approach, experimental results of three partially coherent Schell-model sources are presented and analyzed. A brief discussion of possible applications is provided in closing.

Two different methodologies for generating an electromagnetic Gaussian-Schell model source are discussed. One approach uses a sequence of random phase screens at the source plane and the other uses a sequence of random complex transmittance screens. The relationships between the screen parameters and the desired electromagnetic Gaussian-Schell model source parameters are derived. The approaches are verified by comparing numerical simulation results with published theory. This work enables one to design an electromagnetic Gaussian-Schell model source with pre-defined characteristics for wave optics simulations or laboratory experiments.

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Topical application of EGF presented clinical improvements on dry eye by stabilizing the tear film and maintaining the integrity of epithelium. The results indicate that EGF has potential as a therapeutic agent in clinical treatment of dry eye.