Two neutralizing human scFv, b1 and h12 were identified initially using ELISA,employing highly purified virus as the coating antigen. The biosensor technique based on imaging ellipsometry was employed directly to detect two neutralizing monoclonal antibodies and serial serum samples from 10 SARS patients and 12 volunteers who had not SARS. Further, the kinetic process of interaction between the antibodies and SARS-CoV was studied using the real-time function of the biosensor. The biosensor is consistent with ELISA that the antibody h12 showed a higher affinity in encountering the virus than antibody b1. The affinity of antibody b1 and antibody h12 was 9.5 x 10(6) M(-1) and 1.36 x 10(7) M(- 1), respectively. As a label free method, the biosensor based on imaging ellipsometry proved to be a more competent mechanism for measuring serum samples from SARS patients and the affinity between these antibodies and the SARS coronavirus.
Amino-modified tetrapod-like ZnO nanostructures were tried as novel carriers for
mammalian cell transfections. The nanostructures consisted of four needle-shaped tetrahedrally
arranged legs connected at the center. After silica coating and amino modification, ZnO
nanostructures complexes bound plasmid DNA through electrostatic interactions in aqueous solution.
When mixed with cells, DNA-nanostructures attached easily onto cell membranes and entered the
cells for gene expressions. Due to high positive charge densities on surfaces and needle-shaped
tetrahedral structures, functionalized ZnO used as carriers for cell transfections with both high
transfection efficiency and little cytotoxicity. And a possible transfection machamism was proposed
in this report.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations鈥揷itations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.