Investigation of interaction between two neutralizing monoclonal antibodies and SARS virus using biosensor based on imaging ellipsometry

Cai, Qianqian; Duan, Jinzhu; Wang, Zhan Hui; Chen, Yan Yan; Zhang, Pan He; Li, Zhan; Yan, Xi Yun; Cao, Wu Chun; Jin, Gang

doi:10.1007/s10544-006-8305-2

Cited by 54 publications

(35 citation statements)

References 30 publications

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“…Discrete grayscale above the capture antibody control [13] was used as various cut-off level to obtain TP, FN, TN and FP when pathology test was used as reference criterion. The TPF, FPF and Youden's index were then calculated, as shown in Table 3.…”

Section: Preliminary Applicationmentioning

confidence: 99%

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Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry

et al. 2011

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High sensitive and quantitative detection of alpha-fetoprotein (AFP) by biosensor based on imaging ellipsometry (BIE) through biological amplification was investigated. AFP firstly reacted with the rat monoclonal antibody (rat-mAb) initially immobilized on glutaraldehyde modified silicon surface, then rabbit anti-human AFP polyclonal antibodies (Rabbit-pAb) and goat anti-rabbit IgG (goat-IgG) were sequentially applied to amplify signal. Results revealed that signal was enhanced approximately six fold. The linear range of AFP detection was 20.0-200.0 ng/ml with a low limit of 5.0 ng/ml (S/N = 3). The cross-reaction rate was less than 5.2% evaluated by biomarker (carcinoembryonic antigen, carbohydrate 19-9 and carbohydrate antigen 242) and two common proteins (human serum albumin, fibrinogen) and their mixture. Coefficient variation (CV) for intra-slide and inter-slide reproducibility were 10.3%, 6.6%, 6.3% and 10.7%, 7.9%, 6.4% for 41.6 ng/ml, 83.2 ng/ml and 128.4 ng/ml AFP. In clinic application, cut-off value at 28.6 ng/ml was also determined with sensitivity 0.72 and specificity 0.94. Results of 47 clinic patient samples detected by BIE were in good agreement with those of electrochemiluminescence immunoassay (ECLIA) (R2 = 0.9949).The area under receiver operating characteristic curve (ROC) curve is 0.89. Statistical analysis showed that BIE is high agreement with ECLIA (Kappa = 0.733, U = 3.21NU0.01). It shows a potential for hepatocellular carcinoma (HCC) diagnosis.

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Section: Preliminary Applicationmentioning

confidence: 99%

“…Compared with above methods, biosensor based on imaging ellipsometry (BIE) [8] has the advantages of labelfree, rapid, and high through-put analysis [9][10][11]. It has many applications especially in biomedical field [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry

et al. 2011

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“…9,10 Previous work theoretically and empirically verifies that it can be used in many fields of bioassay including specific combination of antigen-antibody, virus examination, and biomarker measurement. [19][20][21][22]31,38 In this study, a one-step immunoassay for CA19-9 utilizing BIE is demonstrated. The results are also compared with ECLIA by correlation analysis.…”

Section: Introductionmentioning

confidence: 99%

A One-Step Immunoassay for Carbohydrate Antigen 19-9 by Biosensor Based on Imaging Ellipsometry

2010

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Abstract-Cancer markers can offer much more information for developing or developed cancers. Simple and shorttime assay of cancer biomarker is essential for clinic diagnosis and management. In this study, a one-step immunoassay for carbohydrate antigen 19-9 (CA19-9) by biosensor based on imaging ellipsometry (BIE) is described and its analysis performances are evaluated. Anti-CA19-9 monoclonal antibody as ligand covalently immobilized on carboxyl-modified silicon substrate and its immobilization concentration concurrently screened. Then, bovine serum albumin (BSA) is used to block for acquisition of sensing layer. The detection linear range is from 15.0 to 120.0 U/mL with a minimum limit of 10.0 U/mL (S/N = 3). The intraslide and inter-slide reproducibility, expressed as coefficient variation (CV%), are 9.9, 5.9, 5.4% and 9.6, 7.6, 5.3% for 31.9, 57.4, and 99.2 U/mL of CA19-9, respectively. Accuracy determined by spiked-recovery is among 95.1-106.7%. Specificity expressed as cross-reaction rate are all below 5.8% evaluated by three analogous biomarkers alphafetoprotein (AFP), carcinoembryonic antigen (CEA), and carbohydrate antigen 242 (CA242). Application of BIE to 25 clinic samples demonstrated that the results are in high correlate with electrochemiluminescent immunoassay (ECLIA, R 2 = 0.997). The proposed immunoassay has the potential for clinic application.

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“…Developed in 1995 (Jin et al, 1996(Jin et al, , 1995, the biosensor based on imaging ellipsometry (BIE) employs a protein analysis technique that combined high spatial resolution imaging ellipsometry with multi-protein microarray. BIE has been mainly applied to biomedical fields, such as (1) biomolecule interaction (Qi et al, 2006a;Wang and Jin, 2003b). (2) Hormone detection (Zhao et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation

et al. 2009

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a b s t r a c tA rapid detection and identification of pathogens is important for minimizing transfer and spread of disease. A label-free and multiplex biosensor based on imaging ellipsometry (BIE) had been developed for the detection of phage M13KO7. The surface of silicon wafer is modified with aldehyde, and proteins can be patterned homogeneously and simultaneously on the surface of silicon wafer in an array format by a microfluidic system. Avidin is immobilized on the surface for biotin-anti-M13 immobilization by means of interaction between avidin and biotin, which will serve as ligand against phage M13KO7. Phages M13KO7 are specifically captured by the ligand when phage M13KO7 solution passes over the surface, resulting in a significant increase of mass surface concentration of the anti-M13 binding phage M13KO7 layer, which could be detected by imaging ellipsometry with a sensitivity of 10 9 pfu/ml. Moreover, atomic force microscopy is also used to confirm the fact that phage M13KO7 has been directly captured by ligands on the surface. It indicates that BIE is competent for direct detection of phage M13KO7 and has potential in the field of virus detection.

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Investigation of interaction between two neutralizing monoclonal antibodies and SARS virus using biosensor based on imaging ellipsometry

Cited by 54 publications

References 30 publications

Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry

Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry

A One-Step Immunoassay for Carbohydrate Antigen 19-9 by Biosensor Based on Imaging Ellipsometry

Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation

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