In this study, we investigated the interface contacting inhibition behaviors of chitosan against bacterial in the dispersing state. For that purpose, chitosan microspheres (CMs) in the dispersing state was prepared by the emulsification cross-linking method. The CMs had smooth surface and spherical shape with the diameter of about 124 mm. They were stable after sterilization at 121uC and 150 kPa for 20 min. The CMs had similar antibacterial activity to that of chitosan in the solution form. Their antibacterial activities increased with the increase of the CM concentration, while decreased with the increase of pH of the system. It was found that the CMs with the degree of deacetylation (DD) of 63.6% exhibited the highest antibacterial activity, while the CMs with the DD of 83.7% exerted the lowest antibacterial activity among the three tested samples.
The aims of current study were to choose a method for preparing sterile chitosan-a,b-glycerophosphate (CS-a,b-GP) in situ-forming hydrogel which had potential applications in tissue engineering and evaluated its biocompatibility and degradation characteristics. The results of sterilization stability tests indicated that sterile formulations could be obtained by ultraviolet irradiation of CS powders, 0.22 mm filtration of a,b-GP and lactic acid solutions, and sterile preparation of CS-a,b-GP formulations. The obtained sterile CS-a,b-GP formulations showed low hemolysis rates and low BSA adsorption at physiological conditions. When injected in vivo the CS-a,b-GP sol turned into gel implant in situ and could be degraded gradually. A minimal inflammatory reaction which could not be found by macroscopic evaluation was induced after injection and new capillary formation was found around the hydrogel humps, making the CS-a,b-GP hydrogel worthwhile to be considered for tissue engineering and biomedical applications.
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