Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli

Liu, Nan; Chen, Xi Guang; Park, Hyun Jin; Liu, Chen‐Guang; Liu, Chengsheng; Meng, Xiong; Yu, Le

doi:10.1016/j.carbpol.2005.10.028

Cited by 437 publications

(234 citation statements)

References 17 publications

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“…These results were similar to those reported by others; Chitosan could inhibit the growth of E. coli (Liu et al, (2006); Tipparat & Oraphan, (2008) ;Zhuang, et al, 2004). Also similar results observed by (Goy et al, 2016) they demonstrated the growth of E. coli and S. aureus, as measured by turbidity.…”

Section: Rersults and Discussionsupporting

confidence: 92%

“…Furthermore, Chitosan from S. lessoniana (322KD, DDA 85%) reported the maximum inhibition against S. aureus at the highest concentration of 5 mg/ml and minimum inhibition against K. pnemoniae and V. cholera (Liu et al, 2006). This result is agreed with the work of Jeon et al, (2001) reported also the increased antibacterial activity with the increase in the concentration of Chitosan.…”

Section: Rersults and Discussionsupporting

confidence: 88%

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The Antimicrobial Activity of Chitosan and Its Application on Kariesh Cheese Shelf Life

El-Dahma

Khattab

Gouda

et al. 2017

Alexandria Science Exchange Journal

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Section: Rersults and Discussionsupporting

confidence: 92%

Section: Rersults and Discussionsupporting

confidence: 88%

The Antimicrobial Activity of Chitosan and Its Application on Kariesh Cheese Shelf Life

El-Dahma

Khattab

Gouda

et al. 2017

Alexandria Science Exchange Journal

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“…The dietary treatments were as follows (1) control diet (0 ppm COS), (2) control diet plus ,1 kDa COS, (3) control diet plus 3 to 5 kDa COS, (4) control diet plus 5 to 10 kDa COS, (5) control diet plus 10 to 50 kDa COS and (6) control diet plus 50 to 100 kDa COS. The COS was sourced from Kitto Life Co. Ltd (Kyungki-do, Seoul, Korea) and was supplemented in the experimental diets at a concentration of 250 ppm (Helander et al, 2001;Liu et al, 2006). Both the COS content and the deacetylation percentage of all of these COS were .70%.…”

Section: Experimental Dietsmentioning

confidence: 99%

“…Many of the biological properties reported for chitosan and COS depend on MW and concentration Liu et al, 2006;Chung and Chen, 2008). However, the results of studies investigating the biological properties of COS have been inconsistent and this variation is partly due to the widely different MW used across studies (Jeon et al, 2001;Liu et al, 2006). The current study was designed to determine the effects of dietary supplementation of COS at varying MW on pig performance, faecal scoring, selected microbial populations, volatile fatty acid (VFA) concentrations and nutrient digestibility post-weaning.…”

Section: Introductionmentioning

confidence: 99%

The effects of supplementing varying molecular weights of chitooligosaccharide on performance, selected microbial populations and nutrient digestibility in the weaned pig

Walsh

Sweeney

Bahar

et al. 2013

Animal

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An experiment (complete randomised design) was conducted to investigate the effects of supplementing different molecular weights (MW) of chitooligosaccharide (COS) on pig performance, selected microbial populations and nutrient digestibility post-weaning. A total of 396 weaned piglets (24 days of age, 7.3 kg 6 (s.d.) 1.7 kg live weight) were assigned to one of six dietary treatments (22 replicates/ treatment) for a 33-day experimental period. The dietary treatments were as follows (1) control diet (0 ppm COS), (2) control diet plus ,1 kDa COS, (3) control diet plus 3 to 5 kDa COS, (4) control diet plus 5 to 10 kDa COS, (5) control diet plus 10 to 50 kDa COS and (6) control diet plus 50 to 100 kDa COS. The COS were included at 250 ppm in the diets. There was no significant effect of dietary treatment on piglet performance during the starter period (days 0 to 18; P . 0.05). However, there were quadratic responses in both daily gain (P , 0.05) and gain to feed ratio (P , 0.05) to the increased MW of COS inclusion during the weaner period (days 18 to 33) with all COS-supplemented treatments improving daily gain and gain to feed ratio compared with the control. There was a quadratic response in faecal scoring to the increased MW of COS inclusion from days 0 to 7 (P , 0.001), days 7 to 14 (P , 0.001) and during the overall experimental period (P , 0.01) with all the COS-supplemented treatments having an improved faecal score compared with the control. During the weaner period, there was a cubic response in lactic acid bacteria and Escherichia coli populations as the MW of COS increased (P , 0.05). The 5 to 10 kDa and 10 to 50 kDa COS increased lactic acid bacteria populations compared with the control, whereas lactic acid bacteria populations decreased at 50 to 100 kDa. The 5 to 10 kDa, 10 to 50 kDa and 50 to 100 kDa COS decreased E. coli populations compared with the control. There was a cubic response in the apparent total tract digestibility of dry matter (DM; P , 0.01), organic matter (OM; P , 0.01), ash (P , 0.01), nitrogen (N; P , 0.01) and gross energy (GE; P , 0.01) to the increased MW of COS inclusion during the weaner period. The 5 to 10 kDa COS had a higher apparent total tract digestibility of DM, OM, ash, N and GE in comparison to the control, whereas the apparent total tract nutrient digestibility of these nutrients decreased at 10 to 50 kDa. The current results indicate that the MW ranges of 5 to 10 kDa and 10 to 50 kDa COS decreased E. coli numbers while increasing nutrient digestibility of the diets.

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“…Attempts have been made to increase its solubility and bioavailability through encapsulation in biodegradable polymeric nanoparticles (Vemula et al 2006;Salmaso et al 2007). Chitosan (CS), the deacetylated form of chitin (2-amino-2-deoxy-(1-4)-Dglucopyranan), exhibits excellent biodegradability, biocompatibility and antimicrobial activity (Liu et al 2006); thus, CS nanoparticles are extensively used to deliver hydrophobic compounds including drugs, vitamins, proteins, nutrients and phenolics into the biological systems (Hu et al 2008;Jang and Lee 2008). CS acts as a wall/shell material to envelop these compounds bearing multiple negative charges via cationic crosslinking to generate stable, non-toxic, biodegradable nanosized particles (Keawchaoon and Yoksan 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

et al. 2015

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Ferulic acid (FA) is a widely distributed hydroxycinnamic acid found in various cereals and fruits exhibiting potent antioxidant and anticancer activities. However, due to low solubility and permeability, its availability to biological systems is limited. Non-toxic chitosan-tripolyphosphate pentasodium (CS-TPP) nanoparticles (NPs) are used to load sparingly soluble molecules and drugs, increasing their bioavailability. In the present work, we have encapsulated FA into the CS-TPP NPs to increase its potential as a therapeutic agent. Different concentrations of FA were tested to obtain optimum sized FA-loaded CS-TPP nanoparticles (FA/CS-TPP NPs) by ionic gelation method. Nanoparticles were characterized by scanning electron microscopy, Fourier transformation infrared spectroscopy (FTIR), thermogravimetric analyses and evaluated for their anticancer activity against ME-180 human cervical cancer cell lines. The FTIR spectra confirmed the encapsulation of FA and thermal analysis depicted its degradation profile. A concentration-dependent relationship between FA encapsulation efficiency and FA/ CS-TPP NPs diameter was observed. Smooth and spherical FA-loaded cytocompatible nanoparticles with an average diameter of 125 nm were obtained at 40 lM FA conc. The cytotoxicity of 40 lM FA/CS-TPP NPs against ME-180 cervical cancer cell lines was found to be higher as compared to 40 lM native FA. Apoptotic morphological changes as cytoplasmic remnants and damaged wrinkled cells in ME-180 cells were visualized using scanning electron microscopic and fluorescent microscopic techniques. Data concluded that chitosan enveloped FA nanoparticles could be exploited as an excellent therapeutic drug against cancer cells proliferation.

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Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli

Cited by 437 publications

References 17 publications

The Antimicrobial Activity of Chitosan and Its Application on Kariesh Cheese Shelf Life

The Antimicrobial Activity of Chitosan and Its Application on Kariesh Cheese Shelf Life

The effects of supplementing varying molecular weights of chitooligosaccharide on performance, selected microbial populations and nutrient digestibility in the weaned pig

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

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