The objectives of this study were to determine the involvement of Oxidative Stress (OS) in the pathogenesis of dengue hemorrhagic fever (DHF) through the analysis of oxidative stress Index (OSI). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activity, and OSI were measured in 61 child dengue patients and (aged 6 months-18 years) with three different stages of DHF, i.e stage I, II, and III. The results show that the levels of MDA, SOD and CAT activity, and OSI significantly different between the group. The all parameters that investigated in this present study seems higher MDA level and OSI in the higher grade of DHF, except for SOD and CAT activity. From this result, it can be concluded that oxidative stress pathways might be involved in the pathomechanism of DHF and OSI might be used as a biomarker for OS and the severity in DHF patients.
BACKGROUND: Coal dust is known to trigger hypersensitivity and inflammation of the respiratory tract as it increases oxidative stress leading to asthma. To date, the relationship of coal dust exposure in the pathomechanism of asthma remains unclear. This study was aimed to examine the effect of coal dust exposure on the superoxide dismutase (SOD) activity and the oxidative DNA damage indicated by increased serum 8-hydroxy-2' -deoxyguanosine (8-OHdG) in asthmatic mice.METHODS: Twenty-four female balb/c mice were divided into four groups. The first group was the control group. The second group was the negative control group which composed of mice exposed to coal dust particles. The third group was composed of ovalbumin (OVA)-sensitized mice. The fourth group was composed of OVA-sensitized mice and exposed to coal dust particles. The inflammatory process was identified by serum interleukin (IL)-13 concentration using Enzyme-linked Immunosorbent Assay (ELISA) method. Meanwhile, the oxidative stress was examined by measuring the SOD activity using the Nitro Blue Tetrazolium (NBT) method, and the 8-OHdG concentration was quantified by ELISA method.RESULTS: There was an increasing IL-13 in OVAtreated coal dust exposed group along with the increment of 8-OHdG (statistically not significant). SOD activity measured in serum was decreased in all groups (p>0.05). Combination of OVA and coal dust showed the worst effect on IL-13, 8-OHdG and SOD activity.CONCLUSION: Coal dust exposure for four weeks does not adequately induce the oxidative DNA damage in asthmatic mice.KEYWORDS: asthma, coal dust, IL-13, SOD, 8-OHdG
This present study was undertaken to investigate the effect of cadmium (Cd) exposure on an increasing risk of Diabetes Melitus (DM), through the measurement of blood glucose level and liver glucokinase activity in rats. The subjects that used in this study are 15 male rats (Rattus novergicus) with normal activity, 2-3 months old, and weighing 300±10 gram. The research subjects the divided into 3 groups; P0 group are given commercial fed rats diets only; P1 are given commercial fed rats + Cd with a concentration of 3 mg/l in drinking water for 1 day (acute); and P2 are given commercial fed rats+Cd with a concentration of 3 mg/l in drinking water for 4 weeks (subacute). The results of this present study shows that treatment with Cd significantly increase the levels of blood glucose (P < 0,05). The result also showed that treatment with Cd can increase the Km value of liver glucokinase, and it means Cd exposure can decrease the affinity between glucose and glucokinase. The present study demonstrated that Cd exposure could increase the risk of DM through increased the blood glucose and decrease the activity of liver glucokinase. Keywords: Cadmium, glucose metabolism, glucose, glucokinase
<p><strong>ABSTRAK</strong></p><p><strong>Pendahuluan:</strong> Jahe merah dan jintan hitam merupakan tanaman yang sudah lama digunakan untuk minuman herbal yang bermanfaat untuk kesehatan. Akan tetapi belum banyak masyarakat yang mengetahui cara pembuatannya. Berkaitan dengan tersebut, dilakukan pelatihan kepada ibu-ibu kelompok pengajian Langgar Ar-Rahman Banjarbaru dengan tujuan memberikan edukasi dan ketrampilan cara pembuatan minuman kesehatan.</p><p><strong>Metode:</strong> Metode yang digunakan adalah induktif partisipatif secara kelompok, yang diikuti oleh 37 anggota kelompok pengajian. Pemberian materi melalui penyuluhan dan pelatihan. Materi yang diberikan mulai pemilihan bahan hingga membuat minuman kesehatan yang siap disajikan.</p><p><strong>Hasil dan Pembahasan: </strong>Kegiatan pelatihan pembuatan minuman kesehatan berbahan jahe merah dan jintan hitam berjalan dengan lancar. Para peserta antusias dalam mendengarkan pemaparan materi, dan aktif dalam tanya jawab seputar pembuatan pembuatan minuman kesehatan. Selain itu juga termotivasi untuk meningkatkan kesehatan dengan membuat dan mengonsumsi minuman kesehatan berbahan jahe merah dan jintan hitam.</p><p><strong>Kesimpulan:</strong> kegiatan ini adalah ibu-ibu anggota pengajian Langgar Ar-Rahman termotivasi untuk membuat dan mengonsumsi minuman kesehatan berbahan jahe merah dan jintan hitam.</p><p> </p><p>Kata kunci: jahe merah; jintan hitam; minuman kesehatan</p><p> <strong>ABSTRACT</strong></p><p><strong>Introduction:</strong> Red ginger and nigella sativa have long been used for herbal drinks that are beneficial for health. However, many people know how to make it. So that training is needed for members of the Langgar Ar-Rahman Banjarbaru recitation group. The aim is to provide education and skills on how to make health drinks.</p><p><strong>Method: </strong>The method used is participatory inductive in groups. The training was attended by 37 members of the study group. The material is given counseling and training. The material provided starts from the selection of ingredients to making health drinks that are ready to be served.</p><p><strong>Results and Discussion:</strong> The training activities ran smoothly. Participants are serious and actively participate in activities. In addition, they are also motivated to improve their health by making and consuming health drinks made from red ginger and black cumin.</p><p><strong>Conclusion:</strong> this activity is that the members of the Langgar Ar-Rahman recitation are motivated to make and consume health drinks made from red ginger and black cumin.</p><p> </p><p>Keywords: red ginger; black cumin; healthy drink</p>
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