Highlights d SARS-CoV-2 nucleocapsid mutations R203K/G204R associate with B.1.1.7 (Alpha) emergence d R203K/G204R variants possess a replication advantage over the preceding lineages d R203K/G204R variants show enhanced infectivity and disease severity in the hamster model

In addition to the mutations on the spike protein (S), co-occurring mutations on nucleocapsid (N) protein are also emerging in SARS-CoV-2 world widely. Mutations R203K/G204R on N, carried by high transmissibility SARS-CoV-2 lineages including B.1.1.7 and P.1, has a rapid spread in the pandemic during the past year. In this study, we performed comprehensive population genomic analyses and virology experiment concerning on the evolution, causation and virology consequence of R203K/G204R mutations. The global incidence frequency (IF) of 203K/204R has rose up from nearly zero to 76% to date with a shrinking from August to November in 2020 but bounced later. Our results show that the emergence of B.1.1.7 is associated with the second growth of R203K/G204R mutants. We identified positive selection evidences that support the adaptiveness of 203K/204R variants. The R203K/G204R mutant virus was created and compared with the native virus. The virus competition experiments show that 203K/204R variants possess a replication advantage over the preceding R203/G204 variants, possibly in relation to the ribonucleocapsid (RNP) assemble during the virus replication. Moreover, the 203K/204R virus increased the infectivity in a human lung cell line and induced an enhanced damage to blood vessel of infected hamsters' lungs. In consistence, we observed a positive association between the increased severity of COVID-19 and the IF of 203K/204R from in silicon analysis of global clinical and epidemic data. In combination with the informatics and virology experiment, our work suggested the contribution of 203K/204R to the increased transmission and virulence of the SARS-CoV-2. In addition to mutations on the S protein, the mutations on the N protein are also important to virus spread during the pandemic.

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African swine fever is a widespread and highly contagious disease in the porcine population, which is caused by African swine fever virus (ASFV). The PCR and ELISA detection methods are the main conventional diagnostic methods for ASFV antigen/antibody detection in the field. However, these methods have limitations of expensive equipment, trained technicians, and time-consuming results. Thus, a rapid, inexpensive, accurate and on-site detection method is urgently needed. Here we describe a double-antigen-sandwich lateral-flow assay based on gold nanoparticle-conjugated ASFV major capsid protein p72, which can detect ASFV antibody in serum samples with high sensitivity and specificity in 10 min and the results can be determined by naked eyes. A lateral flow assay was established by using yeast-expressed and acid-treated ASFV p72 conjugated with gold nanoparticles, which are synthesized by seeding method. A high coincidence (97.8%) of the assay was determined using clinical serum compared to a commercial ELISA kit. In addition, our lateral flow strip can detect as far as 1:10,000 diluted clinically positive serum for demonstration of high sensitivity. In summary, the assay developed here was shown to be rapid, inexpensive, accurate and highly selective. It represents a reliable method for on-site ASFV antibody detection and may help to control the ASFV pandemic.

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In this paper we study the persistence of lower dimensional hyperbolic invariant tori for generalized Hamiltonian systems. Here the generalized Hamiltonian systems refer to the systems which may admit a distinct number of action and angle variables. In particular, systems under consideration can be odddimensional. Under Rüssmann-type non-degenerate condition, by introducing a modified linear KAM iterative scheme, we proved that the majority of the lower-dimensional hyperbolic invariant tori persist under small perturbations for generalized Hamiltonian systems.

In this paper, we consider the periodic solutions of a variable coefficient wave equation which models the forced vibrations of a nonhomogeneous string and the propagation of seismic waves in nonisotropic media. Under Dirichlet–Neumann boundary conditions, we find some important properties for the variable coefficient wave operator. Then, based on these properties, we obtain the existence and multiplicity of periodic solutions by using the Leray–Schauder degree theory.

In this paper, we give some simple criteria of non-integrability and partial integrability in a Laurent polynomial ring C[u ± 1 , · · · , u ± n ] for general semi-quasihomogeneous systems. (2000). 58F07, 34A20. Mathematics Subject Classification