2021
DOI: 10.1101/2021.05.24.445386
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Nucleocapsid mutation R203K/G204R increases the infectivity, fitness and virulence of SARS-CoV-2

Abstract: In addition to the mutations on the spike protein (S), co-occurring mutations on nucleocapsid (N) protein are also emerging in SARS-CoV-2 world widely. Mutations R203K/G204R on N, carried by high transmissibility SARS-CoV-2 lineages including B.1.1.7 and P.1, has a rapid spread in the pandemic during the past year. In this study, we performed comprehensive population genomic analyses and virology experiment concerning on the evolution, causation and virology consequence of R203K/G204R mutations. The global inc… Show more

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Cited by 12 publications
(16 citation statements)
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“…ORF3a is an essential protein for viral cytotoxicity and is known to activate the inflammasome. This mutation causes a dramatic change in protein structure, affecting the binding affinity of Orf3a–S and Orf3a–Orf8 protein interactions, and finally may be correlated with enhanced viral virulence (Banoun 2021, Wu et al 2021). This dramatic change in protein structure is also reported for S194L and R203K/G204R in Nucleocapsid.…”
Section: Resultsmentioning
confidence: 99%
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“…ORF3a is an essential protein for viral cytotoxicity and is known to activate the inflammasome. This mutation causes a dramatic change in protein structure, affecting the binding affinity of Orf3a–S and Orf3a–Orf8 protein interactions, and finally may be correlated with enhanced viral virulence (Banoun 2021, Wu et al 2021). This dramatic change in protein structure is also reported for S194L and R203K/G204R in Nucleocapsid.…”
Section: Resultsmentioning
confidence: 99%
“…The R203/G204 amino acids are located in the serine/arginine-rich (SR-rich) linker region of Nucleocapsid, which is involved in modulating Nucleocapsid multimerization, and cellular localization. Therefore, R203K/G204R mutations may destabilize the protein, followed by a decrease in the overall structural flexibility, and finally can increase the virulence of SARS-CoV-2 (Leary et al 2021, Rahman et al 2021, Troyano-Hernáez et al 2021, Wu et al 2021)…”
Section: Resultsmentioning
confidence: 99%
“…Recently, other noteworthy mutations of the nucleocapsid of the SARS-CoV-2 have been detected and studied (Badua et al, 2021;Rahman et al, 2021). As an example, the mutation 203K/204R has been demonstrated to have competitive advantages as well as increased infectivity and virulence (Wu et al, 2021).…”
Section: Discussionmentioning
confidence: 99%
“…The immunological mechanisms concerning the nucleocapsid and the possible effects that the changes of this protein can have on transmissibility, replicability and pathogenicity of the virus are still mostly unclear. Recently, evidence has been found supporting the role played by certain nucleocapsid aminoacidic substitutions in promoting higher diffusivity, infectivity and virulence of SARS-CoV-2 (Wu et al, 2021).…”
Section: Introductionmentioning
confidence: 99%
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